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大鼠肺泡巨噬细胞对人胚肺成纤维细胞增殖的抑制作用

张坚松, 于凤江, 瞿树林, 李翔

湖南医学高等专科学校生理学教研室.湖南,长沙 410006

摘要

实验采用[~(3)H]TdR掺入标记法测定微量培养人胚肺成纤维细胞的增殖,观察到健康大鼠的肺泡巨噬细胞(AM)可抑制成纤维细胞增殖。经调理的酵母多糖激活后,AM的抑制作用加强;而经消炎痛处理的AM,抑制作用转为被促进增殖作用所取代;测定AM上清液中前列腺素E(PGE)含量,显示其抑制作用与PGE含量相关。结果提示,AM有抑制和促进肺成纤维细胞增殖的双重作用,正常时以抑制作用占优势;PGE可能是AM产生的主要的肺纤维化抑制因子。

关键词： 肺泡巨噬细胞; 细胞培养; 细胞增殖; 前列腺素E; 肺纤维化

Inhibitory effect of alveolar macrophages on the proliferation of pulmonary fibroblasts

Zhang Jiansong, Yu Fengjiang, Qu Shulin, Li Xiang

Department of Physiology,Hunan Medical School.Changsha 410006,Hunan

Abstract

The effect of alveolar macrophages (AM) harvested from Wistar rats by lung lavage on proliferation of human embryo pulmonary fibroblasts in culture was investigated. It was observed that supernatants of AM decreased the uptake of [~(3)H] TdR by teh pulmonary fibroblasts. The AM activated with opsonized zymosan (OPZ) showed a stronger inhibitory effect on fibroblast proliferation compared with inactivated AM. Following pretreatment with indomethacin, the inhibitory effect of AM was abolished and reversed to stimulatory effect on pulmonary fibroblast proliferation. The PGE content in AM supernatant was measured with radioimmunoassay. It was observed that the inhibitory effect of AM was highly correlated to prostaglandin (PGE) content in the supernatant of AM.

Key words: Alveolar macrophage;Pulmonary fibrosis;Cell culture;Proliferation;Prostaglandins E

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引用本文：

张坚松, 于凤江, 瞿树林, 李翔. 大鼠肺泡巨噬细胞对人胚肺成纤维细胞增殖的抑制作用[J]. 生理学报 2002; 54 (3): .

Zhang Jiansong, Yu Fengjiang, Qu Shulin, Li Xiang. Inhibitory effect of alveolar macrophages on the proliferation of pulmonary fibroblasts. Acta Physiol Sin 2002; 54 (3): (in Chinese with English abstract).