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八肽胆囊收缩素对大鼠心功能的影响及受体机制

赵晓云, 凌亦凌, 孟爱宏, 单宝恩, 张君岚

河北医科大学病理生理学教研室.河北,石家庄 050017；河北医科大学第四医院科研中心.河北,石家庄 050017

摘要

为探讨八肽胆囊收缩素(CCK-8)对麻醉大鼠心功能的影响及受体机制,实验监测了左心室收缩压(LVP)、左心室收缩与舒张期内压变化的最大速率(±LV dp/dt_(max))、心率(HR)和平均动脉压(MAP)。结果如下:小剂量CCK-8(0.4#mu#g/kg)可引起心动过速,MAP、LVP和±LV dp/dt_(max)轻度上升;中剂量CCK-8(4#mu#g/kg)和大剂量CCK-8(40#mu#g/kg)可引起心动过缓,MAP、LVP和±LV dp/dt_(max)显著增加;应用CCK-受体(CCK-R)拮抗剂丙谷胺(1.0mg/kg)抑制以上变化;由逆转录-聚合酶链反应(RT-PCR)检测到心肌组织有CCK-A受体(CCK-AR)和CCK-B受体(CCK-BR)mRNA表达。以上结果提示:CCK-8可激活心肌组织的CCK-R,引起剂量依赖性的心功能增加和心率改变。

关键词： 胆囊收缩素; 心功能; 受体

Effects of cholecystokinin octapeptide on rat cardiac function and the receptor mechanism

Zhao Xiaoyun, Ling Yiling, Meng Aihong, Shan Baoen, Zhang Junlan

Department of Pathophysiology,Hebei Medical University.Shijiazhuang 050017,Hebei；China

Abstract

The aim of this study was to explore the effects of cholecystokinin octapeptide (CCK-8) on cardiac function an the receptor mechanism in anesthetized rats. The mean arterial pressure (MAP), heart rate (HR), the left ventricle systolic pressure (LVP) and the maximal/minimum rate of LVP (±LV dp/dt_(max)) were measured. The results obtained are as follows. (1) Low dose of CCK-8 (0.4#mu#g/kg i.v.) caused tachycardia and slight increase in MAP, LVP and ±LV dp/dt_(max) (P<0.01), while medium dose (4.0 #mu#g/kg i.v.) and high dose of CCK-8 (40#mu#g/kg i.v.) elicited a bradycardia and marked increase in MAP, LVP and ±LV dp/dt_(max) (P<0.01). (2) Proglumide (1.0mg/kg i.v.), a CCK-receptor (CCK-R) antagonist, significantly inhibited the pressor effects of CCK-8, whilst it reversed the bradycardic responses (P<0.01). (3) Using reverse transcription polymerase chain reaction (RT-PCR), CCK-A receptor (CCK-AR) and CCK-B receptor (CCK-BR) mRNA were expressed in myocardium of rats. The above results indicate that CCK-8 may enhance cardiac function in a dose-dependent manner and elicit a change in HR, which is likely induced by the activation of CCK-R on myocardium.

Key words: Cholecystokinin;Cardiac function;Receptor

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引用本文：

赵晓云, 凌亦凌, 孟爱宏, 单宝恩, 张君岚. 八肽胆囊收缩素对大鼠心功能的影响及受体机制[J]. 生理学报 2002; 54 (3): .

Zhao Xiaoyun, Ling Yiling, Meng Aihong, Shan Baoen, Zhang Junlan. Effects of cholecystokinin octapeptide on rat cardiac function and the receptor mechanism. Acta Physiol Sin 2002; 54 (3): (in Chinese with English abstract).