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乙酰胆碱对三种人垂体腺瘤细胞增殖及凋亡的影响

迟素敏, 李承新, 刘亚莉, 朱运龙, 顾建文, 杜亮, 王复周

第四军医大学生理学教研室.陕西,西安 710032；第四军医大学西京医院全军皮肤性病中心.陕西,西安 710032；第四军医大学西京医院脑外科.陕西,西安 710032

摘要

体外培养的人垂体无功能瘤、催乳素瘤和生长激素瘤等三种瘤细胞中均有胆碱酯酶的表达,但明显少于正常垂体;ACh对三种类型的人垂体腺瘤细胞增殖代谢的影响相类似,不同浓度的ACh能明显抑制体外培养的三种人垂体腺瘤细胞的增殖,呈明显的剂量效应关系,同时ACh能减少垂体腺瘤细胞进入S、G_(2)期的细胞比例,而使处于G_(1)期的细胞比例增加;ACh的这种作用可被阿托品阻断,但不受筒箭毒的影响;ACh对体外培养的三种人垂体腺瘤细胞凋亡无明显影响。结果提示,ACh可能以旁分泌或自分泌的方式作用于垂体前叶细胞,对垂体腺瘤细胞增殖分化有调控作用,而且是通过ACh M受体来实现的。

关键词： 乙酰胆碱; 垂体; 腺瘤; 增殖; 凋亡

The effect of acetylcholine on the proliferation and apoptosis of three kinds of cultured human pituitary adenoma cells

Chi Sumin, Li Chengxin, Liu Yali, Zhu Yunlong, Gu Jianwen, u Liang, Wang Fuzhou

Department of Physiology,the Fourth Military Medical University.Xi^an 710032,Shaanxi；China；Department of Neurosurgery,Xijing Hospital,the Fourth Military Medical University.Xi^an 710032,Shaanxi

Abstract

The results showed that (1) the positive staining of ChAT was obviously observed in the cells of the three kinds of human pituitary adenoma, however, it was lower than that in normal human pituitary gland; (2) ACh had a similar effect on the proliferation of the three kinds of human pituitary adenoma cells. ACh at 0.1-10 #mu#mol/L decreased the [~(3)H] TdR incorporation and the MTT A value in a dose-dependent manner. At the same time, ACh decreased the ratio of S or G_(2) phase pituitary adenoma cells significantly, but increased the ratio of G_(1) phase pituitary tumour cells markedly; (3) the effect of acetylcholine on the proliferation of human pituitary adenoma cells was inhibited by atropine, but not by tubocurarine; (4) ACh had no effect on the apoptosis of human pituitary adenoma cells cultured in vitro. These data suggest that ACh may have a significant modulating effect on the proliferation of pituitary adenoma cells by means of paracrine or autocrine, and the effect is mediated by muscarinic receptor.

Key words: Acetylcholine;Pituitary gland;Adenoma;Proliferation;Apoptosis

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引用本文：

迟素敏, 李承新, 刘亚莉, 朱运龙, 顾建文, 杜亮, 王复周. 乙酰胆碱对三种人垂体腺瘤细胞增殖及凋亡的影响[J]. 生理学报 2002; 54 (3): .

Chi Sumin, Li Chengxin, Liu Yali, Zhu Yunlong, Gu Jianwen, u Liang, Wang Fuzhou. The effect of acetylcholine on the proliferation and apoptosis of three kinds of cultured human pituitary adenoma cells. Acta Physiol Sin 2002; 54 (3): (in Chinese with English abstract).