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低浓度内皮素-1对活性氧抑制肺表面活性物质脂质合成的保护

罗自强, 冯丹丹, 周伏文, 张长青, 秦晓群, 孙秀泓

中南大学湘雅医学院生理学教研室.湖南,长沙 410078

摘要

黄嘌呤-黄嘌呤氧化酶超氧阴离子生成系统呈剂量依赖性地降低肺组织~(3)H-胆碱的掺入量;ET-1可减轻活性氧所致~(3)H-胆碱掺入量的减少和肺组织丙二醛含量的增高;但对肺组织超氧化物歧化酶、过氧化氢酶及总抗氧化能力无明显影响;ET-1可分别提高和降低肺组织细胞微粒体和胞浆的CCT活性,并可减轻活性氧所致肺微粒体CCT活性的降低。结果表明,低浓度ET-1具有保护肺微粒体的CCT活性、减轻氧化性肺损伤所致PS合成障碍的作用,其保护机制并非通过影响肺组织内源性抗氧化系统而实现。

关键词： 内皮素1; 肺表面活性物质; CTP:磷酸胆碱二胞苷酰基转移酶; 活性氧

Protective effect of low concentration endothelin-1 on the reactive oxygen-induced inhibition of pulmonary surfactant lipid synthesis

Luo Ziqiang, Feng Dandan, Zhou Fuwen, Zhang Changqing, Qin Xiaoqun, Sun Xiuhong

Department of Physiology,Xiangya Medical College,Central Southern University.Changsha 410078,Hunan

Abstract

These results suggest that ET-1 at low concentration can protect the microsomal CCT activity and reduce the inhibition of PS lipid synthesis induced by oxidant lung injury. The protective mechanism of ET-1 is not relative to the pulmonary endogenous antioxidant defense system.

Key words: Endothelin-1;Pulmonary surfactant;CTP;Phosphoiylcholine cytidylyltransferase

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引用本文：

罗自强, 冯丹丹, 周伏文, 张长青, 秦晓群, 孙秀泓. 低浓度内皮素-1对活性氧抑制肺表面活性物质脂质合成的保护[J]. 生理学报 2002; 54 (2): .

Luo Ziqiang, Feng Dandan, Zhou Fuwen, Zhang Changqing, Qin Xiaoqun, Sun Xiuhong. Protective effect of low concentration endothelin-1 on the reactive oxygen-induced inhibition of pulmonary surfactant lipid synthesis. Acta Physiol Sin 2002; 54 (2): (in Chinese with English abstract).