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脂多糖通过诱导白细胞介素-1的生成引起迷走传入神经活动

路秀英, 杨贵贞, 孙辉臣

解放军白求恩国际和平医院.河北,石家庄 050082；吉林大学基础医学部免疫学教研室.吉林,长春 130021

摘要

为探讨脂多糖(lipopolysaccharide,LPS)引起迷走传入神经活动是否可能通过白细胞介素-1(interleukin-1,IL-1)的作用,将Wistar大鼠随机分为LPS实验组和生理盐水对照组,用免疫组织化学方法检测迷走神经结状神经节c-Fos及CD14的表达以及腹腔迷走神经周围Mac-1阳性巨噬细胞(macrophage,M#phi#)。用L929细胞增殖法检测LPS刺激M#phi#上清IL-1的生物活性。用原位杂交的方法检测迷走神经结状神经节Ⅰ型白细胞介素-1受体(IL-1 RⅠ)mRNA的表达。结果显示,LPS组迷走神经结状神经节神经元c-Fos蛋白表达为阳性,而对照组迷走神经结状神经节神经元c-Fos蛋白表达为阴性。LPS注射后1h,见腹腔迷走神经周围M#phi#数量明显增多。M#phi#在LPS刺激后45min、1h和2h时,IL-1生成明显增高。LPS组迷走神经结状神经节IL-1 RⅠmRNA表达为阳性。

关键词： 迷走神经; cFos; 结状神经节; 白细胞介素1(IL1; 白细胞介素1受体(IL1RⅠ).

The activation of vagus afferent in response to lipopolysaccharide: The role of interleukin-1

Lu Xiuying, Yang Guizhen, Sun Huichen

Norman Bethune National Peace Hospital.Shijiazhuang 050082,Hebei；China

Abstract

To study the possibility of activation of vagus afferent in response to lipopolysaccharide (LPS) through interleukin-1 (IL-1), Wistar rats were randomly divided into LPS group and saline (NS) group. The expression of c-Fos, CD14 and Mac-1 were detected by immunohistochemistry staining. IL-1 bioactivity was determined by L929 cell proliferation. The expression of IL-1R I mRNA was detected by in situ hybridization. Our results showed that there were some c-Fos protein expression positive neurons in the nodose ganglion in LPS group, but no c-Fos protein expression positive neurons in the nodose ganglion in control group. The number of macrophages (M#phi#) in the connective tissue surrounding the abdominal vagus increased significantly in response to LPS i.p. Forty-five minutes after the application of LPS, the IL-1 bioactivity in the supernatant of M#phi# was increased. Positive IL-1 R I mRNA neurons were also observed in the nodose ganglion in the LPS group.

Key words: Vagus nerve;c-Fos;Nodose ganglion;IL-l;IL-l R I

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引用本文：

路秀英, 杨贵贞, 孙辉臣. 脂多糖通过诱导白细胞介素-1的生成引起迷走传入神经活动[J]. 生理学报 2002; 54 (2): .

Lu Xiuying, Yang Guizhen, Sun Huichen. The activation of vagus afferent in response to lipopolysaccharide: The role of interleukin-1. Acta Physiol Sin 2002; 54 (2): (in Chinese with English abstract).