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血管紧张素Ⅱ在小鼠卵泡闭锁中的作用

程勇, 焦丽红, 刘瑞华, 王庆彬, 王红, 夏国良

中科院动物研究所计划生育生殖生物学国家重点实验室.北京 100080；中国农业大学生物学院.北京 100094

摘要

24日龄小鼠给予PMSG(10IU/只)后6d时,卵巢中出现大量闭锁卵泡,颗粒细胞DNA琼脂糖电泳显示了梯形条带;随卵泡闭锁发生,卵巢AngⅡ含量增加;AngⅡ显著拮抗FSH刺激颗粒细胞雌二醇生成的作用。我们认为,AngⅡ参与了对小鼠卵泡闭锁的调节。

关键词： 血管紧张素Ⅱ; 卵泡闭锁; 颗粒细胞; 雌二醇

Effect of angiotensin II on follicular atresia in mouse

Cheng Yong, Jiao Lihong, Liu Ruihua, Wang Qingbin, Wang Hong, Xia Guoliang

State Key Laboratory of Reproductive Biology,Institute of Zoology,Chinese Academy of Sciences.Beijing 100080；China

Abstract

The results showed that: (1)a large number of atretic follicles were found in the ovary of 24-day-old mouse after 6-day treatment of PMSG. Deoxyribonucleic acid (DNA) extracted from granulosa cells clearly showed a ladder band under agarose gel electrophoresis analysis. (2)the contents of Ang II in the ovary extremely increased with the development of follicular atresia. (3)Ang II significantly antagonized the stimulating effect of the follicle-stimulating hormone (FSH) on estradiol (E_(2)) generation of granulosa cells. It is suggested that Ang II may be involved in the regulation of follicular atresia in mouse.

Key words: Angiotensin II;Follicular atresia;Granulosa cells;Estradiol

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引用本文：

程勇, 焦丽红, 刘瑞华, 王庆彬, 王红, 夏国良. 血管紧张素Ⅱ在小鼠卵泡闭锁中的作用[J]. 生理学报 2002; 54 (1): .

Cheng Yong, Jiao Lihong, Liu Ruihua, Wang Qingbin, Wang Hong, Xia Guoliang. Effect of angiotensin II on follicular atresia in mouse. Acta Physiol Sin 2002; 54 (1): (in Chinese with English abstract).