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核磁共振磁场照射对子代大鼠海马突触形态的影响

蒋马莉, 韩太真, 杨东伟, 陈明霞

西安交通大学医学院生理教研室.陕西,西安 710061；西安交通大学医学院电镜室.陕西,西安 710061

摘要

研究观察了孕期磁共振磁场照射对子代大鼠海马突触超微结构的影响。SD孕鼠妊娠d12--18给予0.35T核磁共振(MRI)磁场照射。测量1、2和5月龄雌性仔鼠海马CA1区和齿状回的突触结构参数,用立体计量学方法进行定量测定。结果显示,磁场照射可引起2月龄子代大鼠海马CA1区突触间隙增宽,齿状回突触活性区长度变短、突触界面曲率和活性区面密度减小;5月龄子代大鼠CA1区突触间隙增宽,突触后致密物变薄,突触界面曲率减小,齿状回突触间隙增宽。结果提示,妊娠期接受MRI磁场照射可引起海马突触超微结构的改变。对这些结构变化与行为损害之间的关系进行了讨论。

关键词： 学习; 记忆; 突触; 海马; 孕期暴露的延迟影响; 磁共振成像; 磁场

Morphological alteration of the hippocampal synapses in rats prenatally exposed to magnetic resonance imaging magnetic fields

Jiang Mali, Han Taizhen, Yang Dongwei, Chen Mingxia

Department of Physiology,School of Medicine,Xi^an Jiaotong University.Xi^an 710061,Shaanxi；China

Abstract

When the offspring were 1, 2, or 5-month-old, the synaptic morphologic parameters were measured in female offspring. In the 2-month-old MRI group, the curvature of synaptic interface, the length of active zone and the surface density per unit volume (S_(v)) of active zone in the dentate gyrus (DG) decreased significantly, and the width of synaptic cleft increased in the CA1 area. In the 5-month-old MRI group, the width of synaptic cleft increased, the thickness of postsynaptic density and the curvature of synaptic interface decreased significantly in the CA1 region, and the width of synaptic cleft increased in the DG. No significant change was observed in the 1-month-old group. These results suggest that prenatal exposure to themedical magnetic fields causs synaptic ultrastructural changes. The relationship of these changes with behavioral impairments was discussed.

Key words: Learning;Memory;Synapse;Hippocampus;Delayed effects of prenatal exposure;Magnetic resonance imaging;Magnetic fields

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引用本文：

蒋马莉, 韩太真, 杨东伟, 陈明霞. 核磁共振磁场照射对子代大鼠海马突触形态的影响[J]. 生理学报 2003; 55 (6): .

Jiang Mali, Han Taizhen, Yang Dongwei, Chen Mingxia. Morphological alteration of the hippocampal synapses in rats prenatally exposed to magnetic resonance imaging magnetic fields. Acta Physiol Sin 2003; 55 (6): (in Chinese with English abstract).