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噪声刺激后豚鼠耳蜗抗氧化能力的变化和#alpha#-硫辛酸对声损伤的保护作用

刁明芳, 刘海瑛, 张琰敏, 高文元

上海第二军医大学生理教研室.上海 200433

摘要

(1)无噪声对照组,动物听阈无明显变化;噪声刺激后生理盐水组,听阈上升的幅度明显高于#alpha#-硫辛酸组(P<0.05)。(2)噪声+生理盐水组,TAC明显低于无噪声对照组(P<0.05);噪声+#alpha#-硫辛酸组同噪声+生理盐水组相比,TAC明显升高(P<0.05),同无噪声对照组相比,无显著性差异(P>0.05)。(3)噪声+生理盐水组,NO含量高于无噪声对照组(P<0.05);噪声+#alpha#硫辛酸组同噪声+生理盐水组相比,NO含量明显减少(P<0.05),同无噪声对照组相比,差异无显著性(P>0.05)。上述结果提示,噪声刺激后血清TAC降低,耳蜗组织内NO含量增加;#alpha#-硫辛酸可通过抗氧化机制对噪声性听力损伤(NIHL)发挥保护作用。

关键词： 噪声性听力损伤; 耳蜗; 总抗氧化能力; 一氧化氮; α-硫辛酸

Changes in antioxidant capacity of the guinea pig exposed to noise and the protective effect of #alpha#-lipoic acid against acoustic trauma

Diao Mingfang, Liu Haiying, Zhang Yanmin, Gao Wenyuan

Department of Physiology,Second Military Medical University.Shanghai 200433

Abstract

The threshold of hearing in the control group was relatively stable, while the hearing threshold in the noise+saline group was significantly higher than those in the noise+#alpha#-lipoic acid group (P<0.05). TAC level of the noise+saline group was significantly lower than that of the control group (P<0.05). TAC level of the noise+#alpha#-lipoic acid group was significantly higher than that of the noise+saline group (P<0.05), while there was no significant difference in the levels between the noise+#alpha#-lipoic acid group and the control group (P>0.05). The NO level of the cochlear tissue in the noise+#alpha#-lipoic acid group was significantly higher than that of the group (P<0.05), Cochlear NO level in the noise+#alpha#-lipoic acid group was significantly lower than that of the noise+saline group (P<0.05), while there was no significant difference in cochlear NO levels between the noise+#alpha#-lipoic acid group and the control group (P>0.05). The results obtained indicate that noise exposure causes a decrease in serum TAC and on increase in NO in cochlea. #alpha#-Lipoid acid exerts a protective effect against hearing loss in acoustic trauma through its antioxidant effects.

Key words: Noise-induced hearing loss;Cochlea;Total antioxidant capacity;Nitric oxide;#alpha#-lipoic acid

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引用本文：

刁明芳, 刘海瑛, 张琰敏, 高文元. 噪声刺激后豚鼠耳蜗抗氧化能力的变化和#alpha#-硫辛酸对声损伤的保护作用[J]. 生理学报 2003; 55 (6): .

Diao Mingfang, Liu Haiying, Zhang Yanmin, Gao Wenyuan. Changes in antioxidant capacity of the guinea pig exposed to noise and the protective effect of #alpha#-lipoic acid against acoustic trauma. Acta Physiol Sin 2003; 55 (6): (in Chinese with English abstract).