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海马长时程增强与26S蛋白酶复合体活性变化的关系

胡志安, 谭银玲, 罗峻, 黎海蒂, 李希成

第三军医大学基础医学部生理教研室.重庆 400038；第三军医大学基础医学部微生物教研室.重庆 400038

摘要

强直刺激前,26S蛋白酶复合体活性为190±14.3cpm/(100#mu#g·2h),强直刺激诱导fEPSP斜率增加10min时,其活性升为273±18.3cpm/(100#mu#g·2h),强直刺激诱导fEPSP斜率增加60min时,26S蛋白酶复合体活性又降为210±12.8cpm/(100#mu#g·2h)。NMDA受体特异阻断剂AP-5在损害LTP产生的同时,抑制26S蛋白酶复合体活性升高。实验结果显示:大鼠海马LTP产生过程中,26S蛋白酶复合体活性存在一个短时间的,依赖于N-methyl-D-aspartate(NMDA)受体的升高过程。

关键词： 长时程增强; 蛋白酶复合体; N-methyl-D-aspartate（NMDA）; 受体; 海马; 大鼠

Relationship between hippocampal long term potentiation induction and activity of 26S proteasome

Hu Zhian, Tan Yinling, Luo Jun, Li Haidi, Li Xicheng

Department of Physiology,Third Military Medical University.Chongqing 400038；China

Abstract

26S proteasome activity was 190±14.3 cpm/(100#mu#g·2h) before tetanus, a significant increase in 26S proteasome activity 273±18.3 cpm/(100#mu#g·2h) was found 10 min after tetanus, when the slope of fEPSP was markedly increased. Interestingly, 26S proteasome activity returned to baseline level (210±12.8 cpm/(100#mu#g·2h) 60 min after tetanus. Moreover, the N-methyl-D-aspartate (NMDA) receptor inhibitor AP-5, which blocked LTP, prevented the increase in the 26S proteasome activity. The results suggest that NMDA receptors contribute to the transient increase in 26S proteasome activity during induction of LTP in the hippocampal CA1 region.

Key words: Long term;Potentiation proteasome;N-methyl-D-aspartate （ NMDA）;Receptors;Hippocampus;Rats

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引用本文：

胡志安, 谭银玲, 罗峻, 黎海蒂, 李希成. 海马长时程增强与26S蛋白酶复合体活性变化的关系[J]. 生理学报 2003; 55 (6): .

Hu Zhian, Tan Yinling, Luo Jun, Li Haidi, Li Xicheng. Relationship between hippocampal long term potentiation induction and activity of 26S proteasome. Acta Physiol Sin 2003; 55 (6): (in Chinese with English abstract).