ISSN 0371-0874, CN 31-1352/Q

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外源性重组人睫状神经营养因子抑制或人成肌细胞的体外分化

陈晓萍, 刘红, 刘淑红, 吴燕, 吴海涛, 范明

军事医学科学院基础医学研究所.北京 100850

摘要

为探讨外源性重组人睫状神经营养因子(rhCNTF)在成肌细胞分化中的作用,实验观察了0-10ng/ml rhCNTF对成人成肌细胞体外分化的影响。结果表明,与对照组相比,2.5-10ng/ml rhCNTF能显著抑制成肌细胞的体外分化(P<0.01),并呈量-效依赖关系,且这种抑制作用是可逆的。Western Blot分析提示,这种抑制作用伴有成肌细胞分化期特异标志myogenin和p21表达量的显著降低(P<0.01),以及成肌细胞增殖期特异标志myf5和desmin表达量的显著增加(P<0.01)。因此可以认为,外源性rhCNTF能可逆地抑制成人成肌细胞的体外分化并保持增殖。

关键词: 重组人睫状神经营养因子; 成肌细胞; 分化; 增殖

Exogenous rhCNTF inhibits myoblast differentiation of skeletal muscle of adult human in vitro

Chen Xiaoping, Liu Hong, Liu Shuhong, Wu Yan, Wu Haitao, Fan Ming

Institute of Basic Medical Sciences,Academy of Military Medical Sciences.Beijing 100850

Abstract

To determine the role of recombinant human ciliary neurotrophic factor (rhCNTF) in myogenesis, we observed the effects of rhCNTF (0-10ng/ml) on myoblast differentiation of adult human in vitro. The results showed that compared with the control group, the groups of 2.5-10 ng/ml rhCNTF treatment significantly inhibited myoblast differentiation (P<0.01), and the inhibition was dose-dependent and reversible. Western blots also indicated that compared with the control group, the expression of myogenin and p21, markers of myoblast differentiation phase, was significantly reduced (P<0.01), while Myf5 and desmin, markers of myoblast proliferative phase, significantly increased (P<0.01) in the groups of 2.5-10 ng/ml rhCNTF treatment. These findings demonstrate that exogenous rhCNTF can reversibly inhibit differentiation but permits proliferation of adult human myoblasts in vitro.

Key words: Recombinant human ciliary neurotrophic factor (rhCNTF);Myoblast differentiation;Proliferation;

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引用本文:

陈晓萍, 刘红, 刘淑红, 吴燕, 吴海涛, 范明. 外源性重组人睫状神经营养因子抑制或人成肌细胞的体外分化[J]. 生理学报 2003; 55 (4): .

Chen Xiaoping, Liu Hong, Liu Shuhong, Wu Yan, Wu Haitao, Fan Ming. Exogenous rhCNTF inhibits myoblast differentiation of skeletal muscle of adult human in vitro. Acta Physiol Sin 2003; 55 (4): (in Chinese with English abstract).