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ERK在17#beta#-雌二醇抑制大鼠血管损伤后平滑肌细胞增殖中的作用

王庭槐, 谈智, 付晓东, 杨丹, 胡飞雪, 李永勇

中山大学中山医学院生理教研室.广东,广州 510080

摘要

在去势雌性大鼠中建立颈总动脉球囊损伤模型,实验分单纯去势组(OVX)、去势给予E_(2)治疗组(E_(2)+OVX)、去势后球囊损伤组(OVX+Inj)和去势后球囊损伤给予E_(2)治疗组(E_(2)+OVX+Inj)。分别检测各组血管壁的厚度、血浆中NO的浓度、ERK蛋白表达和活性的变化以及eNOS蛋白表达情况。结果显示,与OVX组相比,OVX+Inj组血浆NO含量明显下降和血管壁厚度明显增厚,E_(2)可增加血浆中NO含量和抑制球囊损伤后血管壁的增厚;E_(2)可以抑制ERK蛋白表达和活化,诱导eNOS蛋白的表达。血浆中NO含量与eNOS蛋白的表达呈正相关,与血管壁厚度和ERK蛋白表达呈负相关。以上结果提示,E_(2)可通过增加血管组织eNOS蛋白表达,促进NO生成,抑制ERK蛋白的表达和活性,从而抑制血管损伤后VSMC的增殖。

关键词： 细胞外信号调节激酶; 17β-雌二醇; 一氧化氮; 血管平滑肌细胞

Effect of ERK on 17#beta#-estradiol-induced inhibition of VSMC proliferation in rats after vascular injury

Wang Tinghuai, Tan Zhi, Fu Xiaodong, Yang Dan, Hu Feixue, Li Yongyong

Department of Physiology,Zhongshan Medical College of Sun Yat-Sen Univrsity.Guangzhou 510080,Guangdong

Abstract

The results showed that compared with OVX, the vessel wall was signifiantly thickened and the plasma content of NO was signficantly decreased in OVX+Inj group, E_(2) significantly decreased the vessel thickness but increased the plasma NO content after balloon injury. E_(2) inhibited the expression of ERK, phosphorylated ERK and induced the eNOS expression. There is a positive correlation between plasma NO content and eNOS protein expression, while there is a negative correlation between plasma NO content and the thickness of vessel. The plasma NO content and the expression of ERK protein were negatively correlated. These results suggest that E_(2) increases the vascular eNOS protein expression and NO release, leading to the inhibition of VSMC proliferation after balloon injury by inhibiting the ERK and phosphorylated ERK protein expression.

Key words: Extracellular signal-regulated kinase;17#beta#-estradiol;Nitric oxide vascular;Smooth muscle cells

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引用本文：

王庭槐, 谈智, 付晓东, 杨丹, 胡飞雪, 李永勇. ERK在17#beta#-雌二醇抑制大鼠血管损伤后平滑肌细胞增殖中的作用[J]. 生理学报 2003; 55 (4): .

Wang Tinghuai, Tan Zhi, Fu Xiaodong, Yang Dan, Hu Feixue, Li Yongyong. Effect of ERK on 17#beta#-estradiol-induced inhibition of VSMC proliferation in rats after vascular injury. Acta Physiol Sin 2003; 55 (4): (in Chinese with English abstract).