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骨桥蛋白增强自发性高血压大鼠血管外膜成纤维细胞的迁移活性

孙爱军, 高平进, 刘建军, 姬开达, 朱鼎良

上海第二医科大学附属瑞金医院上海市高血压研究所.上海 200025

摘要

采用大鼠胸主动脉的培养血管外膜成纤维细胞,用Transwell技术测定培养细胞的迁移能力。用实时定量PCR技术检测mRNA表达。结果表明,在血清和bFGF趋化作用下,SHR培养血管外膜成纤维细胞的迁移活性显著强于WKY(每个视野平均迁移细胞数目,血清:35.20±5.26 vs 22.2±3.27,P<0.05;bFGF:30.23±4.54 vs 19.20±4.47,P<0.05)。进一步研究发现,SHR培养血管外膜成纤维细胞中的骨桥蛋白(OPN)mRNA水平显著高于WKY(1863.23±43.91 vs 326.24±68.29,P<0.01)。反义OPN(100 #mu#mol/L)对血清诱导的SHR血管外膜成纤维细胞迁移有抑制作用(每个视野平均迁移细胞数目38.60±5.98 vs 26.61±3.84, P<0.05)。而正义及错配义OPN组均无此效应。反义OPN对SHR细胞迁移的抑制作用呈浓度依赖性。上述结果证实SHR培养血管外膜成纤维细胞的迁移能力强于WKY,OPN在细胞迁移中可能起重要作用。

关键词： 主动脉; 外膜; 成纤维细胞; 骨桥蛋白; 高血压; 细胞迁移; 重构

Osteopontin enhances migratory ability of cultured aortic adventitial fibroblasts from spontaneously hypertensive rats

Sun Aijun, Gao Pingjin, Liu Jianjun, Ji Kaida, Zhu Dingliang

Shanghai Institute of Hypertension,Ruijin Hospital,Shanghai Second Medical University.Shanghai 200032

Abstract

The results showed that the antisense OPN oligonucleatide significantly inhibited AF migration (cell number per field 38.60±5.98 vs 26.61±3.84, P<0.05), while sense andmismatch OPN oligonucleotide had no effect on cell migration. Therefore, the migration of adventitial fibroblasts appeared to be enhanced in cultures derived from SHR. OPN might be involved in the difference observed.

Key words: aorta;adventitia;Fibroblast;osteopontin;Hypertension;Cell migration;remodeling

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引用本文：

孙爱军, 高平进, 刘建军, 姬开达, 朱鼎良. 骨桥蛋白增强自发性高血压大鼠血管外膜成纤维细胞的迁移活性[J]. 生理学报 2004; 56 (1): .

Sun Aijun, Gao Pingjin, Liu Jianjun, Ji Kaida, Zhu Dingliang. Osteopontin enhances migratory ability of cultured aortic adventitial fibroblasts from spontaneously hypertensive rats. Acta Physiol Sin 2004; 56 (1): (in Chinese with English abstract).