过刊浏览

米非司酮对恒河猴母胎界面细胞凋亡和凋亡分子p53表达的影响

魏鹏, 陶世信, 张学森, 胡召元, 刘以训

中科院动物研究所生殖生物学国家重点实验室.北京 100080

摘要

胎盘形成过程中发生活跃的细胞增殖、迁移和凋亡等活动。p53蛋白是参与调节细胞周期和凋亡过程的原癌基因。实验用原位末端标记、蛋白印迹和免疫组织化学研究正常和米非司酮(RU486)处理后恒河猴母胎界面绒毛和蜕膜组织细胞凋亡及p53蛋白表达。在正常妊娠的恒河猴母胎界面,凋亡信号主要集中在合体滋养层和细胞柱内的一些滋养层细胞;p53蛋白主要定位于细胞滋养层。在母体蜕膜中,也在部分基质细胞中检测到细胞凋亡和p53蛋白表达。经过RU486处理2d后,胎盘绒毛和母体蜕膜中凋亡细胞数都显著增加,绒毛中增加的凋亡信号集中于细胞滋养层。同时,RU486处理也导致绒毛细胞滋养层和蜕膜基质细胞中p53表达明显增加。以上结果提示,在正常妊娠中,生理性的细胞凋亡和p53表达可能是控制细胞滋养层细胞增殖、保持胎盘组织动态平衡的一个重要机制;RU486终止早孕的可能途径之一是促进母胎界面细胞凋亡,推测p53参与这一过程。

关键词： 细胞凋亡; p53; RU486; 恒河猴

Effect of RU486 on apoptosis and p53 expression at the boundary of fetal-maternal interface of rhesus monkey (Macaca mulatta)

Wei Peng, Tao Shixin, Zhang Xuesen, Hu Zhaoyuan, Liu Yixun

State Key Laboratory of Reproductive Biology,Institute of Zoology,Chinese Academy of Sciences.Beijing 100080

Abstract

Western blot analysis showed the specificity of the anti-human antibody used with the monkey tissue. In the placental villi, the apoptotic nuclei were observed mainly in the syncytiotrophoblast and part of the cytotrophoblast within the cell column; p53 protein was detected mainly in the cytotrophoblast. In the endometrium, positive signals for apoptosis and p53 were detected in some stromal cells. After two days of mifepristone treatment, the apoptotic cells increased significantly in both placental villi and endometrium. In the villi, the increased apoptotic nuclei were mainly localized to the cytotrophoblast. At the same time, p53-positive nuclei also increased in both villous cytotrophoblast cells and endometrial stromal cells, after the treatment of RU486. These results suggest that apoptosis and expression of p53 are essential in regulating trophoblastic homeostasis by controlling its proliferation in normal placenta, whereas the up-regulation of p53 protein may play an important role in apoptosis that happens at the fetal-maternal interface induced by RU486.

Key words: apoptosis;p53;RU486; rhesus monkey

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

魏鹏, 陶世信, 张学森, 胡召元, 刘以训. 米非司酮对恒河猴母胎界面细胞凋亡和凋亡分子p53表达的影响[J]. 生理学报 2004; 56 (1): .

Wei Peng, Tao Shixin, Zhang Xuesen, Hu Zhaoyuan, Liu Yixun. Effect of RU486 on apoptosis and p53 expression at the boundary of fetal-maternal interface of rhesus monkey (Macaca mulatta). Acta Physiol Sin 2004; 56 (1): (in Chinese with English abstract).