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海马mu型阿片肽受体介导大鼠癫痫发作敏感性形成

刘辉, 高慧明, 张万琴, 唐一源, 宋鹤山

解放军210医院神经内科.辽宁,大连 116021；大连医科大学生理学教研室.辽宁,大连 116027；大连理工大学神经信息研究所.辽宁,大连 116024

摘要

为探讨海马mu型阿片肽受体介导癫痫发作敏感性形成的作用,实验采用微渗透泵技术,观察大鼠腹侧海马注射mu型阿片肽受体激动剂PL017(2.09、2.59、3.29 #mu#g/#mu#l)、拮抗剂#beta#-funaltrexamine hydrochloride (#beta#-FNA、0.88、1.10、1.35 #mu#g/#mu#l)对红藻氨酸(KA)诱导癫痫发作的干预作用。PL017能够明显缩短癫痫发作潜伏期、增加癫痫发作级别(P<0.05),#beta#-FNA则可显著延长癫痫发作潜伏期、降低发作级别(P<0.01);PL017和#beta#-FNA的干预作用均表现出剂量依赖发作。结果表明,海马mu型阿片肽受体具有促进KA诱导的癫痫发作敏感性形成作用。

关键词： mu opioid receptor; susceptibility to seizures; hippocampus; epilepsy; kainic acid; rat

Effects of chronic administration of PL017 and beta-funaltrexamine hydrochloride on susceptibility of kainic acid-induced seizures in rats

Liu Hui, Gao Huiming, Zhang Wanqin, Tang Yiyuan, Song Heshan

Department of Neurology,PLA 210 Hospital.Dalian 116021,Liaoning；China；Institute of Neuroinformation,Dalian University of Technology.Dalian 116024,Liaoning

Abstract

There is evidence that 5--7d after acute seizure episodes induced by kainic acid (KA) the rats develop a long-lasting increase in the susceptibility to seizures followed by spontaneous recurrent seizures (SRS). The present study was focused on the role of hippocampal mu opioid receptors (MORs) in the susceptibility of rats to seizures with the KA model of epilepsy. The rats received a convulsant dose of KA (10 mg/kg, i.p.) were continuously infused with a selective MOR agonist PL017 (2.09, 2.59, 3.29 #mu#g/#mu#l), or a selective MOR antagonist #beta#-funaltrexamine hydrochloride (#beta#-FNA, 0.88, 1.10, and 1.35 #mu#g/#mu#l) into ventral hippocampus by means of mini-osmotic pumps. Seven days later, the susceptibility of rats to seizures was checkedby a subconvulsant dose of KA (5mg/kg, i.p.). PL017 infusion shortened the latency and increased the stage of seizures induced by subconvulsant dose of KA in a dose-dependent manner. In contrast, infusion of #beta#-FNA exhibited a dose-dependent effect against seizures challenged by subconvulsant dose of KA. These results indicate that hippocampal MOR may exert a promoting effect on the susceptibility of rats to KA-induced seizures.

Key words: mu型阿片肽受体;癫痫发作敏感性;海马;癫痫;红藻氨酸;大鼠

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引用本文：

刘辉, 高慧明, 张万琴, 唐一源, 宋鹤山. 海马mu型阿片肽受体介导大鼠癫痫发作敏感性形成[J]. 生理学报 2004; 56 (1): .

Liu Hui, Gao Huiming, Zhang Wanqin, Tang Yiyuan, Song Heshan. Effects of chronic administration of PL017 and beta-funaltrexamine hydrochloride on susceptibility of kainic acid-induced seizures in rats. Acta Physiol Sin 2004; 56 (1): (in Chinese with English abstract).