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长时程增强诱导和维持过程中海马CA1区神经细胞粘附分子蛋白水平与mRNA表达的变化

胡志安, 谭银玲, 罗峻, 黎海蒂, 李希成, 余争平

第三军医大学生理教研室.重庆 400038；第三军医大学微生物教研室.重庆 400038；第三军医大学劳动卫生教研室.重庆 400038

摘要

既往研究发现,神经细胞粘附分子(NCAM)对海马CA1区突触传递长时程增强(LTP)的诱导和维持极为关键。该文采用原位杂交法和Western blot法,观察了大鼠海马脑片LTP诱导和维持过程中NCAM mRNA和蛋白水平的动态变化过程。结果显示,强直刺激诱发fEPSP斜率升高10min时,海马CA1区NCAM mRNA染色阳性神经元数量显著增加(76.6±11.5个),NCAM蛋白水平亦明显升高(7.190±0.64任意单位/50#mu#g蛋白)。强直刺激诱发fEPSP斜率升高1h时,NCAM mRNA染色阳性神经元数量为73.3±14.0个,NCAM蛋白量为9.031±0.71任意单位/50#mu#g蛋白;与强直刺激后10min比较,NCAM mRNA表达无显著变化,而NCAM蛋白水平变化明显。NMDA受体特异阻断剂AP-5在损害LTP的同时,显著抑制NCAM mRNA和蛋白的增加。实验结果表明,在大鼠海马LTP诱导和维持过程中,NCAM mRNA增强的表达相对稳定,而NCAM蛋白水平呈现先低后高的变化。

关键词： 长时程增强; 神经细胞粘附分子; 海马; 大鼠

Changes in neural cell adhesion molecule mRNA expression and protein level in the CA1 region of the hippocampus during long term potentiation induction and maintenance

Hu Zhian, Tan Yinling, Luo Jun, Li Haidi, Li Xicheng, Yu Zhengping

Department of Physiology,Third Military Medical University.Chongqing 400038；China；Department of Occupational Health,Third Military Medical University.Chongqing 400038

Abstract

The results showed that the number of NCAM mRNA positive labelled neurons significantly increased (76.6±11.5 neurons) 10 min after tetanus when the slope of fEPSP markedly increased. The level of NCAM protein also increased significantly (7.190±0.64 arbitrary unit/50 #mu#g protein) 10 min after tetanus. The number of NCAM mRNA positive labelled neurons no longer changed (73.3±14.0) 1 h after tetanus, however, the NCAM protein level (9.031±0.71) at 1 h after tetanus was higher than that at 10 min after tetanus. Moreover, the NMDA receptor inhibitor AP-5, which blocked LTP, prevented the increase in NCAM mRNA expression and NCAM protein level. The results demonstrate that NCAM mRNA expression maintains a high level, whereas NCAM protein changes from a low level to high level during induction and maintenance of LTP.

Key words: long term potentiation;neural cell adhesion molecules;Hippocampus;Rats

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引用本文：

胡志安, 谭银玲, 罗峻, 黎海蒂, 李希成, 余争平. 长时程增强诱导和维持过程中海马CA1区神经细胞粘附分子蛋白水平与mRNA表达的变化[J]. 生理学报 2004; 56 (1): .

Hu Zhian, Tan Yinling, Luo Jun, Li Haidi, Li Xicheng, Yu Zhengping. Changes in neural cell adhesion molecule mRNA expression and protein level in the CA1 region of the hippocampus during long term potentiation induction and maintenance. Acta Physiol Sin 2004; 56 (1): (in Chinese with English abstract).