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MK-801抑制福尔马林实验引起的大鼠脊髓背角环氧合酶-2的表达

李淑琴, 李文斌, 孙晓彩, 李清君, 陈晓玲, 艾洁

河北医科大学基础医学研究所病理生理学研究室.河北,石家庄 050017

摘要

应用免疫组织化学方法,观察鞘内注射N-methyl-D-aspartate(NMDA)受体拮抗剂MK-801对福尔马林实验引起的大鼠脊髓背角环氧合酶-2(COX-2)表达的影响。结果表明:MK-801对福尔马林实验引起的第1相缩足反射仅有一定抑制作用,但对第2相缩足反射有显著的抑制作用,且呈剂量依赖性。与这种行为学的变化相对应,MK-801可显著抑制福尔马林实验引起的脊髓背角COX-2表达的增加,并且这种抑制作用与MK-801的剂量呈正相关。这些结果表明,在福尔马林实验中,NMDA受体的活动是引起脊髓背角COX-2表达增加的原因之一。

关键词： 环氧合酶-2(COX-2); NMDA受体; MK-801; 甲醛; 脊髓背角

MK-801 inhibits formalin-induced cyclooxygenase-2 expression in the dorsal horn of the spinal cord in rats

Li Shuqin, Li Wenbin, Sun Xiaocai, Li Qingjun, Chen Xiaoling, Ai Jie

Department of Pathophysiology,Institute of Basic Medicine,Hebei Medical University.Shijiazhuang 050017,Hebei

Abstract

The results showed that intrathecal injection of MK-801 significantly inhibited the increase of COX-2 expression in the spinal dorsal horn induced by the formalin injection in a dose-dependent manner, suggesting that the activation of NMDA receptor is one of the mechanisms for the formalin-induced increase of COX-2 expression in the spinal dorsal horn.

Key words: cyclooxygenase-2;N-methyl-D-aspartate receptors;MK-801;formalin;spinal cord dorsal horn

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引用本文：

李淑琴, 李文斌, 孙晓彩, 李清君, 陈晓玲, 艾洁. MK-801抑制福尔马林实验引起的大鼠脊髓背角环氧合酶-2的表达[J]. 生理学报 2004; 56 (1): .

Li Shuqin, Li Wenbin, Sun Xiaocai, Li Qingjun, Chen Xiaoling, Ai Jie. MK-801 inhibits formalin-induced cyclooxygenase-2 expression in the dorsal horn of the spinal cord in rats. Acta Physiol Sin 2004; 56 (1): (in Chinese with English abstract).