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DMSO对悬浮培养的东北红豆杉细胞凋亡和紫杉醇合成的影响

葛志强, 李景川, 元英进, 胡宗定

天津大学化工学院制药工程系.天津 300072

摘要

研究了不同浓度的DMSO对悬浮培养的东北红豆杉(Taxus cuspidata)细胞的增殖能力、细胞活性以及紫杉醇合成和释放等方面的影响,同时应用荧光指示剂双染法检测了细胞凋亡的发生情况。结果显示2%的DMSO处理能显著降低细胞活性,抑制细胞的增殖能力,使细胞核内DNA含量减少,培养中、后期在荧光显微镜下可见部分细胞核出现典型的凋亡形态,同时伴有紫杉醇产量的明显增加;对照组及1%以下浓度组未出现上述改变。结果表明一定浓度的DMSO能诱导细胞凋亡,促进细胞紫杉醇合成能力的提高。

关键词： 东北红豆杉细胞; 二甲基亚砜; 凋亡; 细胞活力; 紫杉醇

Effect of DMSO on apoptosis and taxol biosynthesis in suspension cultured Taxus cuspidata cells

Ge Zhiqiang, Li Jingchuan, Yuan Yingjin, Hu Zongding

Department of Pharmaceutical Engineering,School of Chemical Engineering and Technology,Tianjin University.Tianjin 300072

Abstract

The results showed that 2% DMSO could decrease the cell viability significantly, suppress cell proliferation, and reduce the DNA content in the nucleus. Some of the cells treated with 2% DMSO showed typical apoptotic morphological changes under fluorescence microscope from the 8th to the 27th day of culture (Plate I-b,c,d). In the same time, the yield of taxol was enhanced evidently. These changes in the above description were not observed in the control group and the groups treated by DMSO of concentrations 0.5% and 1%. These findings show that DMSO at a certain concentration induces apoptosis and promotes taxol biosynthesis by Taxus cuspidata cells in suspension culture.

Key words: Taxes cuspidata cell;Dimethyl sulfoxide;Apoptosis;Cell viability;Taxol

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引用本文：

葛志强, 李景川, 元英进, 胡宗定. DMSO对悬浮培养的东北红豆杉细胞凋亡和紫杉醇合成的影响[J]. 生理学报 2001; 53 (6): .

Ge Zhiqiang, Li Jingchuan, Yuan Yingjin, Hu Zongding. Effect of DMSO on apoptosis and taxol biosynthesis in suspension cultured Taxus cuspidata cells. Acta Physiol Sin 2001; 53 (6): (in Chinese with English abstract).