过刊浏览

玉米幼胚高效再生系统的建立

吴敏生, 黄健秋, 卫志明

中科院上海植物生理研究所.上海 200032

摘要

玉米幼胚高效再生植株与下列因素有关:玉米基因型、幼胚大小、幼胚长芽至分化时间、6-BA、IBA、Gelrite。不同品种玉米再生能力有显著差异,幼胚大小在1-2mm之间再生能力强,幼胚长芽至分化时间4-6d最好。激素6-BA浓度在0.5-0.6mg/L之间有利于微芽形成小植株,IBA浓度在0.6-1.0mg/L促进生根。Gelrite可代替琼脂粉用于玉米生根。

关键词： 玉米; 幼胚; 组织培养; 再生植株

Establishment of highly efficient regeneration system of maize

Wu Minsheng, Huang Jianqiu, Wei Zhiming

Shanghai Institute of Plant Physiology,Chinese Academy of Sciences.Shanghai 200032

Abstract

The regeneration of maize immature embryo is related to the following factors: maize genetype, length of immature embryo, the time from budding to differentiation, the presence and concentration of 6-BA, IBA and Gelrite. Different maize varieties have different regeneration capacities, the best combination is that length of immature embryo is 1-2 mm, the time from buding to differentiation is 4-6 days, 6-BA is 0.5-0,6mg/L, IBA is 0.6-1.0mg/L for root growth. Gelrite could replace agar for maize rooting.

Key words: Maize;Immature embryo;Tissue culture;Plant regeneration

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

吴敏生, 黄健秋, 卫志明. 玉米幼胚高效再生系统的建立[J]. 生理学报 2001; 53 (6): .

Wu Minsheng, Huang Jianqiu, Wei Zhiming. Establishment of highly efficient regeneration system of maize. Acta Physiol Sin 2001; 53 (6): (in Chinese with English abstract).