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电融合获得用于选择抗CTV砧木的酸橙与甜橙体细胞杂种植株

刘继红, 邓秀新

华中农业大学作物遗传改良国家重点实验室.湖北,武汉 430070

摘要

在已知参数条件下,通过电场诱导酸橙(Citrus aurantium L.)叶肉原生质体和沙漠蒂甜橙(C. sinensis Osbeck cv. Shamouti)的胚性愈伤组织原生质体融合,融合产物经培养再生出40棵植株。染色体检查表明所得到的植株具有36条染色体,为四倍体植株。再生植株具有翼叶,叶片厚,表现出多倍体的特征。采用2个10-碱基随机引物鉴别再生植株的杂种特性。在2个引物的扩增带型中,再生植株的随机扩增带图里出现了融合亲本的特征带。对再生植株染色体计数和RAPD分析的结果表明它们是酸橙和甜橙种间异源四倍体体细胞杂种植株。这些体细胞杂种植株的获得为选择具有酸橙优良性状、同时抗CTV的新型砧木提供了好的试材。

关键词： 酸橙; 甜橙; 电融合; 四倍体; 体细胞杂种; 柑桔速衰病

Production of somatic hybrid plants between sour orange and sweet orange via electrofusion for creation of CTV-resistant rootstock

Liu Jihong, Deng Xiuxin

National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University.Wuhan 430070,Hubei

Abstract

Leaf-derived protoplasts of sour orange (Citrus aurantium L.) were electrically fused with embryogenic protoplasts of sweet orange (C. sinensis Osbeck cv. Shamouti) based on fusion parameters as previously reported. Forty plants have been obtained from fusion products. Chromosome counting showed that the plants were tetraploids with 36 chromosomes. The plants have thick petiole-winged leaves, indicative of the characteristics of polyploid. Two 10-bp random primers were utilized to identify the hybridity of the plants. Bands specific to fusion parents were detected in the amplification band patterns of the regenerated hybrid plants for the primers. Chromosome counting and RAPD analysis demonstrated that interspecific allotetraploid somatic hybrid plants have been successfully recovered between sour organge and sweet orange. The regeneration of the somatic hybrid plants between sour orange and sweet orange provides good experimental materials for selecting novel rootstock possessing superior traits of sour orange coupled with CTV resistance.

Key words: Sour orange;Sweet orange;Electrofusion;Tetraploid;Somatic hybrid;Citrus tristeza virus

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引用本文：

刘继红, 邓秀新. 电融合获得用于选择抗CTV砧木的酸橙与甜橙体细胞杂种植株[J]. 生理学报 2001; 53 (6): .

Liu Jihong, Deng Xiuxin. Production of somatic hybrid plants between sour orange and sweet orange via electrofusion for creation of CTV-resistant rootstock. Acta Physiol Sin 2001; 53 (6): (in Chinese with English abstract).