转人肝刺激物质基因的肝癌细胞增殖状态研究
安威, 杜海军, 陈莉
首都医科大学细胞生物系.北京 100054
摘要
将人HSS基因导入BEL-7402肝癌细胞,用Northern和Southern杂交法证实该基因在靶细胞中有稳定表达。并通过测定细胞生长曲线、细胞S期比例和细胞MAPK活性,观察到转HSS基因BEL-7402细胞增殖发生了改变。实验结果提示,HSS表达的肝癌细胞DNA合成增加、增殖速度加快,可能与MAPK激活有关。HSS基因表达可促进细胞增殖。
Increased cellular proliferation in BEL-7402 hepatoma cells transfected by human hepatic stimulator substance gene
An Wei, Du Haijun, Chen Li
Department of Cell Biology,Capital University of Medical Sciences.Beijing 100054
Abstract
It is concluded that the introduction of HSS gene into hepatoma cells might be able to stimulate directly the cellular growth in vitro, allowing a possibility of revaluation of HSS mechanism in intact cells.
Key words: Hepatic stimulatory substance;Gene transfection;Liver proliferation
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引用本文:
安威, 杜海军, 陈莉. 转人肝刺激物质基因的肝癌细胞增殖状态研究[J]. 生理学报 2001; 53 (6): .
An Wei, Du Haijun, Chen Li. Increased cellular proliferation in BEL-7402 hepatoma cells transfected by human hepatic stimulator substance gene. Acta Physiol Sin 2001; 53 (6): (in Chinese with English abstract).