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皮质酮快速激活PC12细胞中p38丝列原激活的蛋白激酶

李晓煜, 邱俭, 肖林, 朱剑琴, 陈宜张

南京大学生物科学与技术系.江苏,南京 210093；第二军医大学神经科学研究所.上海 200433；第二军医大学生理学教研室.上海 200433

摘要

实验旨在研究糖皮质激素快速、非基因组作用的细胞内信号传导机制。Western分析研究结果表明,皮质酮可快速激活PC12细胞中p38丝列原激活的蛋白激酶(mitogen-activated protein kinase,MAPK),时间、浓度曲线均为钟形,最大激活为10~(-9) mol/L和15min。糖皮质激素受体阻断剂RU38486不能阻断此作用,而小牛血清白蛋白耦联的皮质酮也能快速激活p38。受体酪氨酸激酶阻断剂genistein对此作用无影响,表明此快速作用不涉及受体酪氨酸激酶活性。此作用能被蛋白激酶C(protein kinase C,PKC)激动剂PMA模拟,而被PKC阻断剂Go6976所阻断。结果表明,皮质酮可能通过推测的膜受体以PKC依赖的方式快速激活p38 MAPK。

关键词： 皮质酮; 非基因组作用; p38; 蛋白激酶C; PC12

Rapid activation of p38 mitogen-activated protein kinase by corticosterone in PC12 cells

Li Xiaoyu, Qiu Jian, Xiao Lin, Zhu Jianqin, Chen Yizhang

Department of Biological Science and Technology,Nanjing University.Nanjing 210093,Jiangsu；China；Department of Physiology,Second Military Medical University.Shanghai 200433

Abstract

The present study using immunoblot showed that corticosterone (B) could induce a rapid activation of p38 in PC12 cells. The dose- and time-response curves were bell-shaped with a maximal activation at 10~(-9)mol/L and 15 min respectively. The activation was not affected by steroid nuclear receptor antagonist RU38486. Bovine serum albumin coupled B (B-BSA) could induce phosphorylation of p38. Tyrosine kinase inhibitor genistein failed to block the phosphorylation, a fact suggesting that the tyrosine kinase activity is not involved in the pathway. On the other hand, phorbol 12-myristate 13-acetate (PMA), a protein kinase C (PKC) activator, could mimic the actions of B, while Goo6976, a PKC inhibitor, could completely abolish the phosphorylation induced by B. These results clearly demonstrate that B activates p38 MAPK readily via a putative membrane receptor through a PKC-dependent pathway.

Key words: Corticosterone;Nongenomication;p38;Protein kinase C(PKC);PC12 cells

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引用本文：

李晓煜, 邱俭, 肖林, 朱剑琴, 陈宜张. 皮质酮快速激活PC12细胞中p38丝列原激活的蛋白激酶[J]. 生理学报 2001; 53 (6): .

Li Xiaoyu, Qiu Jian, Xiao Lin, Zhu Jianqin, Chen Yizhang. Rapid activation of p38 mitogen-activated protein kinase by corticosterone in PC12 cells. Acta Physiol Sin 2001; 53 (6): (in Chinese with English abstract).