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用差异显示PCR法筛选与血管外膜细胞表型转化相关的基因

孙爱军, 高平进, 刘建军, 姬开达, 朱鼎良

上海第二医科大学附属瑞金医院上海市高血压研究所.上海 200025

摘要

为筛选血管外膜成纤维细胞(adventitial fibroblast,AF)与肌成纤维细胞(myofibroblast,MF)间表型转化有关的基因,实验建立了大鼠胸主动脉AF和MF两种细胞模型,用差异显示聚合酶链反应(DD-PCR)技术获得表达差异片段,对差异片段进行克隆和测序分析,并用定量PCR和Northern blot对差异显示结果进行验证。用反义核酸转染技术观察骨桥蛋白(osteopontin,OPN)对AF迁移的影响。

关键词： 血管外膜; 成纤维细胞; 肌成纤维细胞; 差异显示聚合酶链反应; 反义技术

Identification of genes related to cell phenotypic transition by differential display analysis

Sun Aijun, Gao Pingjin, Liu Jianjun, Ji Kaida, Zhu Dingliang

Shanghai Institute of Hypertension,Ruijin Hospital,Shanghai Second Medical University.Shanghai 200025

Abstract

This study suggests that the downregulation of gene encoding NADH dehydrogenase subunit 5 and upregulation of osteopontin gene and several other unknown genes may be involved in the phenotypic transition of adventitial fibroblasts to myofibroblats. Inhibition of the expression of OPN may play an important role in the process of vascular remodeling.

Key words: Adventitia;Fibroblasts;Myofibroblasts;Differential display PCR antisense ;

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引用本文：

孙爱军, 高平进, 刘建军, 姬开达, 朱鼎良. 用差异显示PCR法筛选与血管外膜细胞表型转化相关的基因[J]. 生理学报 2001; 53 (6): .

Sun Aijun, Gao Pingjin, Liu Jianjun, Ji Kaida, Zhu Dingliang. Identification of genes related to cell phenotypic transition by differential display analysis. Acta Physiol Sin 2001; 53 (6): (in Chinese with English abstract).