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白细胞介素-10对血管一氧化氮/一氧化氮合酶系统的影响

夏春芳, 霍勇, 薛林, 朱国英, 唐朝枢

北京大学第一医院心内科.北京 100034

摘要

细菌脂多糖(LPS)呈浓度依赖性地激活诱导型NOS(iNOS),促进NO生成。IL-10(10~(-10)-10~(-8) g/ml)呈浓度依赖性地上调内皮型NOS(eNOS)蛋白表达及其活性,但对iNOS活性及表达无明显影响。IL-10(10~(-9)-10~(-8) g/ml)显著抑制10#mu#g/ml LPS诱导的NO生成和iNOS激活;而高浓度IL-10(10~(-7) g/ml)则上调iNOS的活性,对eNOS蛋白的表达和活性无明显影响。

关键词： 白细胞介素-10; 一氧化氮; 一氧化氮合酶

Influence of interleukin-10 on nitric oxide/nitric oxide synthase system of the aorta

Xia Chunfang, Huo Yong, Xue Lin, Zhu Guoying, Tang Chaoshu

Cardiovascular Department,the First Hospital,Peking University.Beijing 100034

Abstract

To study the influence of the inflammatory factor-interleukin-10 on nitric oxide (NO) and nitric oxide synthase system of rat aorta, Griess assay, production of ~(3)H-citrulline and Western blot were used to determine the release of NO, and the activity and expression of nitric oxide synthase, respectively.

Key words: Interleukixr 10;Nitric oxide;nitric oxide synthase

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引用本文：

夏春芳, 霍勇, 薛林, 朱国英, 唐朝枢. 白细胞介素-10对血管一氧化氮/一氧化氮合酶系统的影响[J]. 生理学报 2001; 53 (6): .

Xia Chunfang, Huo Yong, Xue Lin, Zhu Guoying, Tang Chaoshu. Influence of interleukin-10 on nitric oxide/nitric oxide synthase system of the aorta. Acta Physiol Sin 2001; 53 (6): (in Chinese with English abstract).