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影响豌豆Rubisco组装的因子及其作用机制

陈根云, 肖元珍, 李立人

中科院上海植物生理研究所.上海 200032

摘要

低浓度的羟自由基能降解Rubisco全酶中的大亚基,产生专一的37kD降解多肽片段。该文利用这个特性用低浓度羟自由基对存在于LMW蛋白组分中组装中间体里的大亚基进行专一性的降解,从而对高等植物Rubisco组装的影响因子的作用机制作进一步探讨。

关键词： Rubisco; 组装; 中间体; 羟自由基; 低分子量蛋白组分; 构象

The factors affecting Rubisco assembly and the reaction mechanism in pea

Chen Genyun, Xiao Yuanzhen, Li Liren

Shanghai Institute of Plant Physiology,Chinese Academy of Sciences.Shanghai 200032

Abstract

The process of assembly was inhibited apparently by the presence of K~(+) (5 mmol/L) in the assay medium, and was inhibited almost completely (-90%) when the concentration of K~(+) reached 40 mmol/L. Assembly was not inhibited when K~(+) was replaced by Na~(+) at the same concentration. It shows that K~(+) is able to trigger the changes in LS conformation which hinders the assembly process.

Key words: Rubisco;Asse mbly;Intermediates;Hydroxyl radical;low-molecular weight(LMW)protein incomponents;Confor mation

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引用本文：

陈根云, 肖元珍, 李立人. 影响豌豆Rubisco组装的因子及其作用机制[J]. 生理学报 2001; 53 (5): .

Chen Genyun, Xiao Yuanzhen, Li Liren. The factors affecting Rubisco assembly and the reaction mechanism in pea. Acta Physiol Sin 2001; 53 (5): (in Chinese with English abstract).