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超干贮藏榆树种子萌发过程中ATP和可溶性糖含量的变化

汪晓峰, 景新明, 林坚, 郑光华, 杨世杰, 朱诚, 曾广文

中科院植物研究所.北京 100093；中国农业大学生物学院.北京 100094；浙江大学生命科学学院.浙江,杭州 310029

摘要

经过超干(种子含水量3.73%)贮藏(普通室温下2个月)的榆树种子萌发过程中ATP与可溶性糖含量和ATP酶、淀粉酶、抗氧化酶的活性都高于对照(种子含水量9.34%,普通室温下贮藏),说明适度的超干贮藏有利于保持种子活力。

关键词： 超干贮藏; 种子萌发; ATP; ATP酶; 抗氧化酶

The content change of ATP and soluble sugar of ultradry Ulmus pumila seed during germination

Wang Xiaofeng, Jing Xinming, Lin Jian, Zheng Guanghua, Yang Shijie, Zhu Cheng, Zeng Guangwen

Institute of Botany,Chinese Academy of Sciences.Beijing 100093；China；College of Life Science,Zhejiang University.Hangzhou 310029,Zhejiang

Abstract

During germination of ultradry (UD) Ulmus pumila seed stored for 2 months, ATP content was higher than that of control, which provided enough energy for seedling growth. The result of standard lead precipitation procedures indicated that ATPase activity of UD Ulmus pumila seed was higher, compared with the control seed. The study also demonstrated that UD seed can maintain higher amylase activity and carbohydrate metabolism. The higher activity of anti-oxidation enzyme indicated a higher vigour level of ultradry (UD) Ulmus pumila seed and higher self defense ability against the attak of free radicals than that of control. These results demonstrated that UD seeds could prserve seed vigor during storage, so suitable storage will benefit for seed vigor.

Key words: Ultradry seed storage;Seed germination;ATP;ATPase;Anti- oxidation enzyme

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引用本文：

汪晓峰, 景新明, 林坚, 郑光华, 杨世杰, 朱诚, 曾广文. 超干贮藏榆树种子萌发过程中ATP和可溶性糖含量的变化[J]. 生理学报 2001; 53 (5): .

Wang Xiaofeng, Jing Xinming, Lin Jian, Zheng Guanghua, Yang Shijie, Zhu Cheng, Zeng Guangwen. The content change of ATP and soluble sugar of ultradry Ulmus pumila seed during germination. Acta Physiol Sin 2001; 53 (5): (in Chinese with English abstract).