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脱水速率对黄皮胚轴脱水敏感性及膜脂过氧化的影响

伍贤进, 宋松泉, 钱春梅, 陈玲, 傅家瑞

中山大学基因工程教育部重点实验室与生命科学学院.广东,广州 510275

摘要

在脱水过程中,胚轴的萌发率、活力指数、电解质渗漏速率,超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性逐渐降低,膜脂过氧化产物MDA的含量不断增加。脱水速率愈快,胚轴的半致死含水量就愈低。快速干燥的胚轴能在较低的含水量下存活是因为缩短了在中间含水量下发生的膜脂过氧化作用的时间,以及保持较高的SOD、POD和CAT活性;缓慢干燥的胚轴当与周围环境达到水分平衡后,生活力的丧失将与保持在水分平衡后的时间有关。因此,脱水速率是一种影响顽拗性种子或者胚轴脱水敏感性的重要因子。

关键词： 黄皮胚轴; 脱水速率; 膜脂过氧化; 顽拗性种子; 生活力

Effects of drying at different rates on desiccation sensitivity and membrane lipid peroxidation in Chinese wampee [Clausena lansium (Lour.) Skeels] axes

Wu Xianjin, Song Songquan, Qian Chunmei, Chen Ling, Fu Jiarui

The Key Laboratory of Gene Engineering of Ministry of Education,School of Life Sciences,Zhongshan University.Guangzhou 510275,Guangdong

Abstract

It has been found that the more rapidly the dehydration of axes is achieved, the lower the water content at which 50% of axes were killed by dehydration is. Flash-dried axes can survive lower water contents because the time when aqueous-based membrane lipid peroxidation occurred was significantly shortened at intermediate water contents and higher activities of SOD, POD and CAT remained at lower water contents. After the equilibrium water contents between slowly-dried axes and ambient relative humidity were reached, the loss of viability were associated with the duration the axes are kept at that water content. The dehydration at different rates, therefore, is an important factor which influences desiccation sensitivity of recalcitrant seeds or axes.

Key words: Clausena lansium axis;Drying rate;Membrane lipid peroxidation;Recalcitrant seed;Viability

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引用本文：

伍贤进, 宋松泉, 钱春梅, 陈玲, 傅家瑞. 脱水速率对黄皮胚轴脱水敏感性及膜脂过氧化的影响[J]. 生理学报 2001; 53 (5): .

Wu Xianjin, Song Songquan, Qian Chunmei, Chen Ling, Fu Jiarui. Effects of drying at different rates on desiccation sensitivity and membrane lipid peroxidation in Chinese wampee [Clausena lansium (Lour.) Skeels] axes. Acta Physiol Sin 2001; 53 (5): (in Chinese with English abstract).