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茎用芥菜细胞质雄性不育系子叶原子质体培养和高效植株再生

陈利萍, 张明方, Yutaka H, 曹家树, 陈竹君

浙江大学农业与生物技术学院园艺系.浙江,杭州 310029；日本东京农工大学农学部研究生院.东京 183-8509

摘要

利用茎用芥菜细胞质雄性不育系原生质体培养获得了再生植株,并研究了影响原生质体培养的因素。结果表明,子叶是茎用芥菜原生质体培养最佳的外植体,10d苗龄的子叶原生质体在改良MS培养基上培养3d后发生第1次细胞分裂,6d后发现第2次分裂,3周后形成细胞团,5周后形成肉眼可见的小愈伤。培养基中缺少NAA或2,4-D都会降低愈伤组织的再生能力。在含一定浓度的NAA(0.25mg/L)和2,4-D(0.25mg/L)培养基上诱导的愈伤组织质地致密且有光泽,芽的分化能力高;在MS+BA 1mg/L+NAA 0.2mg/L的培养基上芽的分化频率高达近29%,再生芽在1/2MS+NAA 0.1mg/L培养基上生根,形成完整植株。

关键词： 茎用芥菜; 细胞质雄性不育系; 原生质体培养; 植株再生

Efficient plant regeneration from cotyledon-derived protoplasts of cytoplasmic male-sterile tuber mustard (Brassica juncea Coss. var. tumida Tsen et Lee)

Chen Liping, Zhang Mingfang, Hirata Yutaka, Cao Jiashu, Chen Zhujun

Department of Horticulture,College of Agriculture and Biotechnology,Zhejiang University.Hangzhou 310029,Zhejiang；Japan

Abstract

The NAA or 2, 4-D was necessary for callus proliferation. The absence of NAA and 2, 4-D resulted in production of the callus with low regenerative potential, and in the presence of NAA (0.25 mg/L) and 2, 4-D (0.25 mg/L), compact and bright calli were produced with high reproductive potential. Regenerated shoots were obtained at a frequency of nearly 29% when the compact and bright calli were selected and transferred at an early stage to the medium containing BA (1 mg/L) and NAA (0.2 mg/L). All the regenerated shoots were rooted on 1/2 MS medium with NAA 0.1 mg/L added.

Key words: Tuber mustard;Cytoplasmic male-sterile line;Protoplast culture;Regeneration

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引用本文：

陈利萍, 张明方, Yutaka H, 曹家树, 陈竹君. 茎用芥菜细胞质雄性不育系子叶原子质体培养和高效植株再生[J]. 生理学报 2001; 53 (5): .

Chen Liping, Zhang Mingfang, Hirata Yutaka, Cao Jiashu, Chen Zhujun. Efficient plant regeneration from cotyledon-derived protoplasts of cytoplasmic male-sterile tuber mustard (Brassica juncea Coss. var. tumida Tsen et Lee). Acta Physiol Sin 2001; 53 (5): (in Chinese with English abstract).