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家兔脑干呼吸相关结构向斜方体后核的轴突投射--CB-HRP逆行示踪研究

李勤, 宋刚

山东大学医学院生理教研室.山东,济南 250012

摘要

实验在10只成年家兔上进行。斜方体后核(RTN)内微量注入霍乱毒素#beta#亚单位耦合辣根过氧化酶(CB-HRP)后,在脑桥Kolliker-Fuse核、臂旁内侧核及臂旁外侧核观察到大鼠HRP标记神经元。在延髓孤束核腹外侧区、疑核和后疑核、面神经后核的腹侧及内侧区观察到少数HRP标记神经元。在面神经后核、疑核及后疑核区域观察到大量HRP顺行标记末梢纤维。实验结果表明:RTN和脑桥及延髓的呼吸相关结构之间存在纤维联系。

关键词： 斜方体后核; Klliker-Fuse核; 臂旁内侧核; CB-HRP; 呼吸

Afferent projection to the retrotrapezoid nucleus from respiratory related structures in the brainstem of rabbit--A retrograde CB-HRP tracing study

Li Qin, Song Gang

Department of Physiology,Medical College,Shandong University.Jinan 250012,Shandong

Abstract

Experiments were performed on 10 adult rabbits. After microinjection of cholera toxin B-subunit conjugated horseradish peroxidase (CB-HRP) into the retrotrapezoid nucleus (RTN), retrogradely labeled neurons were mainly observed in the Kolliker-Fuse nucleus (KF), the medial parabrachial nucleus (PBM) and the lateral parabrachial nucleus (PBL). A few labeled neurons were also observed in the ventrolateral solitary tract nucleus, the ambiguus and retroambiguus nuclei, and the ventromedial area of retrofacial nucleus. Labeled terminal fibers were mainly observed in the retrofacial nucleus, the ambiguus and retroambiguus nuclei, the ventrolateral area of solitary tract nucleus, and the PBL-PBM-KF. The above results show that the RTN has axonal connections with the pontine and medullary respiratory related structures.

Key words: Retrotrapezoid nucleus;Kolliker Fuse nucleus;Medial parabrachial nucleus;Horseradish peroxidase;Respiration

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引用本文：

李勤, 宋刚. 家兔脑干呼吸相关结构向斜方体后核的轴突投射--CB-HRP逆行示踪研究[J]. 生理学报 2001; 53 (5): .

Li Qin, Song Gang. Afferent projection to the retrotrapezoid nucleus from respiratory related structures in the brainstem of rabbit--A retrograde CB-HRP tracing study. Acta Physiol Sin 2001; 53 (5): (in Chinese with English abstract).