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过量铜、锌对外生菌根菌牛乳牛肝菌生物量、呼吸和糖酵解酶活性的影响

黄艺, 陶澍

北京大学城市和环境学系.北京 100871

摘要

在过量铜或锌胁迫下,独立培养的外生菌根菌牛乳牛肝菌(Suillus bovinus)的生长和生理活动都受到影响。在添加不同浓度水平的铜或锌的培养基中,菌丝斑直径增长与对照相比显著减小。4周后,菌丝生物量与铜、锌处理浓度呈负指数关系,对菌丝的半致死浓度分别为铜6.5mg/L和锌120mg/L。取半致死浓度和4倍半致死浓度的铜,1/4半致死浓度和半致死浓度的锌处理菌丝,其呼吸强度表现为下降趋势,且己糖激酶和磷酸果糖激酶活性随营养液中铜或锌的增加而降低,丙酮酸激酶活性则随铜或锌浓度的增加而增加。生长在高处理浓度的铜、锌营养液中,己糖激酶和磷酸果糖激酶两种关键酶活性受到抑制,使菌丝的生长和呼吸都受到显著抑制。然而,在相对较低浓度锌50mg/L处理下,生物量、呼吸强度和糖酵解关键酶活性变化不显著,说明S. bovinus的菌丝对锌不敏感。

关键词： 外生菌根菌; 牛乳肝菌; 铜; 锌; 糖酵解; 酶活性

Effects of excessive Cu or Zn on biomass, respiration and activities of glycolytic enzymes in mycelia of ectomycorrhizal fungus Suillus bovinus

Huang Yi, Tao Shu

Department of Urban and Environmental Research,Peking University.Beijing 100871

Abstract

The result showed that heavy metal stress could inhibit biomass and respiration of mycelia by inhibiting the activities of major enzymes of glucolisis (HK and PFK). However, under relatively low concentration of Zn treatment (Zn 50 mg/L), biomass growth, inspiration and enzyme activities were not significantly changed over the control, that showed S. bovinus has stronger resistance against zinc.

Key words: Ectomycorrhizal fungus;Suillus bovinus;Copper;Zinc;Glycolysis;Enzymes activity

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引用本文：

黄艺, 陶澍. 过量铜、锌对外生菌根菌牛乳牛肝菌生物量、呼吸和糖酵解酶活性的影响[J]. 生理学报 2001; 53 (4): .

Huang Yi, Tao Shu. Effects of excessive Cu or Zn on biomass, respiration and activities of glycolytic enzymes in mycelia of ectomycorrhizal fungus Suillus bovinus. Acta Physiol Sin 2001; 53 (4): (in Chinese with English abstract).