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小鼠骨髓内皮细胞条件培养液复合FL及TPO对HPP-CFC及CFU-GM增殖的影响

那晓东, 王绮如

湖南医科大学血液生理研究室.湖南,长沙 410078

摘要

通过传代培养小鼠骨髓内皮细胞,收集无血清条件培养液(ECM),并经超滤得到大于10kD的浓缩液,分别观察ECM和大于10kD的浓缩液复合flt3 ligand(FL)及thrombopoietin(TPO)对体外培养HPP-CFC、CFU-GM的影响。结果表明:ECM或大于10kD的浓缩液对HPP-CFC、CFU-GM的生长均有支持作用;FL或/和TPO与ECM或大于10kD的浓缩液合用能加强对HPP-CFC、CFU-GM生长的刺激作用;FL加TPO与ECM或大于10kD的浓缩液合用对HPP-CFC、CFU-GM生长的刺激作用更加明显;选择FL和TPO特异性的引物,用RT-PCR技术未能检测到小鼠骨髓内皮细胞有FL和TPO mRNA的表达。

关键词： 骨髓内皮细胞; 无血清条件培养液; 造血调控; 小鼠

Effects of murine bone marrow endothelial cell conditioned medium in combination with FL and TPO on the growth of HPP-CFC and CFU-GM

Na Xiaodong, Wang Qiru

Research Laboratory of Blood Physiology,Hunan Medical University.Changsha 410078,Hunan

Abstract

In the present study, the effects of murine bone marrow endothelial cell conditioned medium (ECM) combined with flt3 ligand (FL) or/and thrombopoietin (TPO) on the proliferation of HPP-CFC and CFU-GM were investigated. Both ECM and the concentrated retentate of ECM (MW>10 kD) promoted the growth of CFU-GM and HPP-CFC, and this promoting effect was further enhanced by addition of FL or TPO. Using reverse transcriptase-polymerase chain reaction (RT-PCR) tchnique, the expression of FL and TPO mRNA was not found in murine bone marrow endothelial cells.

Key words: Bone marrow endothelial cells;Serum-free conditioned medium;Regulation of hematopoiesis;Mice

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引用本文：

那晓东, 王绮如. 小鼠骨髓内皮细胞条件培养液复合FL及TPO对HPP-CFC及CFU-GM增殖的影响[J]. 生理学报 2001; 53 (4): .

Na Xiaodong, Wang Qiru. Effects of murine bone marrow endothelial cell conditioned medium in combination with FL and TPO on the growth of HPP-CFC and CFU-GM. Acta Physiol Sin 2001; 53 (4): (in Chinese with English abstract).