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转多基因水稻植株的获得

冯道荣, 许新萍, 李宝健

中山大学生物工程研究中心.广东,广州 510275

摘要

将2-3个外源目的基因与筛选标记基因hpt连在同一个载体上,用基因枪法对1个粳稻品种和4个籼稻品种进行了转化。粳稻中花8号转化频率最高为56.4%,籼稻七丝软占、特青、奇妙香、九六零转化频率分别为6.4%,2.5%,2.8%和1.7%。分子杂交实验证明,通过两次潮霉素抗性筛选的T_(0)代再生植株100%含有hpt基因,其中70%以上含有外源目的基因的完整表达结构。遗传分析表明,大部分T_(1)代植株中潮霉素抗敏比符合孟德尔3:1的分离规律。

关键词： 水稻; 基因枪法; 转基因水稻植株; 分子分析

Production of rice plants containing multiple transgenes

Feng Daorong, Xu Xinping, Li Baojian

Biotechnology Research Center,Zhongshan University.Guangzhou 510275,Guangdong

Abstract

We constructed two or three target genes and the marker gene hpt into one vector, and transformed them into one indica rice variety and four japonica rice varieties. Among them, the Zhonghua No.8 had the highest transformation rate 56.4%, and the transformation rate of Qisiruanzhan, Teqing, Qimiaoxiang and Jiuliuling is 6.4%, 2.5%, 2.8% and 1.7%. Molecular analysis proved that 100% of the regenerated plants contained hpt transgenes after hygromycin selection for two times, and more than 70% of them contained intact expression construct of foreign target gene. Genetic analysis showed that the hyg~(r)/hyg~(s) ratio in most of T_(1) plants is near 3 to 1, corresponding to Mendelian ratio.

Key words: Rice;Biolistic;Transgenic rice plants;Molecular analysis

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引用本文：

冯道荣, 许新萍, 李宝健. 转多基因水稻植株的获得[J]. 生理学报 2001; 53 (4): .

Feng Daorong, Xu Xinping, Li Baojian. Production of rice plants containing multiple transgenes. Acta Physiol Sin 2001; 53 (4): (in Chinese with English abstract).