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甘蓝型油菜BcNA1基因启动子在转基因烟草中对GUS基因表达的调控

石东乔, 周奕华, 万里红, 刘桂珍, 胡赞民, 陈正华

中科院遗传研究所.北京 100101

摘要

通过PCR扩增,从甘蓝型油菜(Brassica napus)品种H165中克隆了种子贮藏蛋白基因BcNA1的启动子,将此启动子与GUS基因相连构建了植物表达载体,利用农杆菌介导法将其导入烟草。对转基因烟草GUS基因检测分析表明,BcNA1基因启动子能特异地启动GUS基因在种子中的表达;而且GUS酶的活性随着转基因烟草种子的发育而变化,同时还间接证明BcNA1基因的启动子在转基因烟草中的遗传传递方式符合孟德尔遗传定律。

Expression of GUS gene in transgenic tobacco regulated by BcNA1 promoter from Brassica napus

Shi Dongqiao, Zhou Yihua, Wan Lihong, Liu Guizhen, Hu Zanmin, Chen Zhenghua

Institute of Genetics,Chinese Academy of Sciences.Beijing 100101

Abstract

The upstream regulatory region of a seed-specific gene was separated from Brassica napus cv. H15 genomic DNA by polymerase chain reaction. Plant expression vector was constructed by ligating this promoter with GUS gene and transferred into tobacco. GUS analysis made on the transgenic plants indicated that the promoter could drive the expression of GUS gene seed-specifically and the activity of GUS changed with the development of seeds. The results of GUS assay also suggested that the genetic transmission of the BcNA1 promoter conforms to Mendelian laws.

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引用本文：

石东乔, 周奕华, 万里红, 刘桂珍, 胡赞民, 陈正华. 甘蓝型油菜BcNA1基因启动子在转基因烟草中对GUS基因表达的调控[J]. 生理学报 2001; 53 (4): .

Shi Dongqiao, Zhou Yihua, Wan Lihong, Liu Guizhen, Hu Zanmin, Chen Zhenghua. Expression of GUS gene in transgenic tobacco regulated by BcNA1 promoter from Brassica napus. Acta Physiol Sin 2001; 53 (4): (in Chinese with English abstract).