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鸡胚脊髓背角中神经营养活性物质的初步分离和检测

刘耀波, 薛庆善, 肖渝平, 王先荣

军事医学科学院基础医学研究所.北京 100850；中山医科大学生理学教研室.广东,广州 510089；中山医科大学生物学教研室.广东,广州 510089；兰州医学院组织学与胚胎学教研室.甘肃,兰州 730000

摘要

从9d龄鸡胚脊髓背、腹角组织提取液中,用Sephadex G-75凝胶层析法分离出DⅠ和DⅡ,VⅠ和VⅡ各组蛋白质组分,体外培养检测各组分对鸡胚背根节的神经营养活性。结果表明,DⅡ组分具有明显的促背根节神经突起生长的作用,而DⅠ组分无此作用;经SDS-PAGE电泳检测,DⅡ组分中蛋白质的分子量范围是61-15kD。而腹角的分离组分VⅠ和VⅡ对背根神经节神经突起的生长并无明显作用。

关键词： 脊髓背角; 神经营养活性; 背根神经节

Preliminary separation and identification of the neurotrophic substances from dorsal spinal cord of chicken embryos

Liu Yaobo, Xue Qingshan, Xiao Yuping, Wang Xianrong

The Institute of Basic Medical Sciences,Academy of Military Medicine.Beijing 100850；China；Department of Biology,Sun Yat-Sen University of Medical Sciences.Guangzhou 510089,Guangdong

Abstract

Extracts of spinal dorsal horn and ventral horn of chicken embryos hatched for 9 days were separated by Sephadex G-75 gel filtration, and different chromatographic fractions of D I and D II, V I and V II were obtained. The neurite-outgrowth promoting effects of the various fractions were assayed by the dorsal root ganglion (DRG) culture in vitro. It was shown that D II, but not D I, fraction of the dorsal horn extract exhibited a significant neurite-outgrowth promoting effect on DRG. By means of SDS-PAGE, the molecular weight of D II fraction ranged from 61 to 15 kD. V I and V II fraction of ventrla horn showed no obvious effect on neurit-outgrowth of DRG in vitro.

Key words: Spinal dorsal horn;Neurotrophic activity;Dorsal root ganglion

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引用本文：

刘耀波, 薛庆善, 肖渝平, 王先荣. 鸡胚脊髓背角中神经营养活性物质的初步分离和检测[J]. 生理学报 2001; 53 (4): .

Liu Yaobo, Xue Qingshan, Xiao Yuping, Wang Xianrong. Preliminary separation and identification of the neurotrophic substances from dorsal spinal cord of chicken embryos. Acta Physiol Sin 2001; 53 (4): (in Chinese with English abstract).