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胆碱脱氢酶基因的转化及转基因番茄耐盐性的鉴定

王淑芳, 王峻岭, 赵彦修, 张慧

山东师范大学逆境植物实验室.山东,济南 250014

摘要

采用PCR方法从E. coli TG1菌株中扩增得到胆碱脱氢酶(Choline dehydrogenase,CDH)基因(betA),其氨基酸编码序列与文献中的资料一致,且能在原核表达载体pBV221中正确表达,并表现出活性。构建该基因的植物表达框架pRbA,经农杆菌介导叶圆盘法转化番茄,对转基因番茄的PCR、Southern、RT-PCR分析证实betA已整合到番茄基因组中,转基因番茄的耐盐性高于对照番茄。

关键词： 胆碱脱氢酶基因; 转基因番茄; 耐盐性

Transformation of choline dehydrogenase gene and identification of salt-tolerance in transgenic tomato

Wang Shufang, Wang Junling, Zhao Yanxiu, Zhang Hui

Key Laboratory of Plant Stress,Shandong Normal University.Jinan 250014,Shandong

Abstract

The betA gene which encodes choline dehydrogenase that converts choline into glycinebetaine has been cloned from Escherichia coli TG1. Agrobacterium-mediated transformation has been used to introduce the betA gene under the control of the 35S promoter of cauliflower mosaic virus as well as the npt II marker gene into tomato (Lycopersicon esculentum). Salt tolerance assays were performed on two independent transgenic plants. Under salt stress, the osmotic adjustment ability of the transgenic tomato was higher than that of the control plant. At 200 mmol/L NaCl, there is no visible symptoms of salt damage in transgenic tomato whereas the lower leaves of the control plant turned yellow and exhibited necrosis. The results suggest a positive effect of the betA gene on the level of salt tolerance in transgenic plants.

Key words: Choline dehydrogenase gene( betA);Transgenic tomato;Salt tolerance

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引用本文：

王淑芳, 王峻岭, 赵彦修, 张慧. 胆碱脱氢酶基因的转化及转基因番茄耐盐性的鉴定[J]. 生理学报 2001; 53 (3): .

Wang Shufang, Wang Junling, Zhao Yanxiu, Zhang Hui. Transformation of choline dehydrogenase gene and identification of salt-tolerance in transgenic tomato. Acta Physiol Sin 2001; 53 (3): (in Chinese with English abstract).