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高温加速老化过程中甜菜种子线粒体小分子量热激蛋白22的变化

宋松泉, Fredlund K M, Moller I M

中山大学生命科学学院.广东,广州 510275；Department of Seed Physiology,Novartis Seeds AB,Box 302,S-26123 Landskrona,Sweden；Department of Plant Physiology,Lund University,Box 117,S-22100 Lund,Sweden

摘要

甜菜种子的人工老化(30℃,100% RH)导致种子的干重、生活力和活力逐渐丧失,线粒体的呼吸速率、细胞色素c氧化酶和苹果酸脱氢酶的活性下降。线粒体蛋白质随着种子老化而显著变化,小分子量热休克蛋白(LMW HSP)22从老化的0d到30d增加,然后从30d到90d下降。可以认为甜菜种子人工老化过程中线粒体活性和LMW HSP 22的含量变化与种子活力密切相关。

关键词： 人工老化; 甜菜; 细胞色素c氧化酶; 苹果酸脱氢酶; 线粒体小分子量热休克蛋白22; 呼吸速率; 种子活力

Changes in low-molecular weight heat shock protein 22 of mitochondria during high-temperature accelerated ageing of Beta vulgaris L. seeds

Song Songquan, Fredlund K M, Moller I M

School of Life Sciences,Zhongshan University.Guangzhou 510275,Guangdong；Sweden

Abstract

High-temperature accelerated ageing (at 30deg C and 100% RH) of sugar beet (Beta vulgaris L. cv. Loke) seeds resulted in radual loss of dry weight, viability and vigour of seeds, and lowering of mitochondrial respiratory rate and activities of cytochrome c oxidase and malate dehydrogenase in mitochondria. Protein composition in mitochondria showed marked changes with ageing of seeds; low-molecular weight heat shock protein (LMW HSP) 22 increased from 0 d to 30 d of ageing, and then decreased from 30 d to 90 d of ageing. It was found that changes in mitochondria activity and LMW HSP 22 content were closely correlated with seed vigour during accelerated ageing of sugar beet seeds.

Key words: Ageing;Beta vulgaris;Cyt .c oxidase;MDH;LMW HSP 22;Respiratory rate;Seed vigour

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引用本文：

宋松泉, Fredlund K M, Moller I M. 高温加速老化过程中甜菜种子线粒体小分子量热激蛋白22的变化[J]. 生理学报 2001; 53 (1): .

Song Songquan, Fredlund K M, Moller I M. Changes in low-molecular weight heat shock protein 22 of mitochondria during high-temperature accelerated ageing of Beta vulgaris L. seeds. Acta Physiol Sin 2001; 53 (1): (in Chinese with English abstract).