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苜蓿抗乙硫氨酸愈伤组织的筛选和特性

徐云远, 贾敬芬

中科院植物研究所.北京 100093；西北大学生物学系.陕西,西安 710069

摘要

用乙硫氨酸为筛选剂,通过幼苗和组织培养筛选得到乙硫氨酸抗性愈伤组织。该愈伤组织在含乙硫氨酸的培养基上表现出较高的半抑制剂量和相对生长量。作为天门冬氨酸途径的产物,甲硫氨酸、异亮氨酸和赖氨酸在所筛选的愈伤组织中分别增加到对照的两倍多,但苏氨酸保持正常水平,另外酪氨酸、半胱氨酸和亮氨酸也有所增加,而在所筛选的愈伤组织中缬氨酸浓度却下降。说明在所筛选愈伤组织中存在一个以上与氨基酸合成相关的酶发生改变。同工酶分析表明,该愈伤组织中出现对照中没有的分子量为44kD的超氧化物歧化酶和分子量为45kD的酯酶谱带。

关键词： 苜蓿; 乙硫氨酸; 同工酶; 抗性系; 组织培养

Selection and characterization of cultured alfalfa calluses resistant to ethionine

Xu Yunyuan, Jia Jingfen

Institute of Botany,Chinese Academy of Sciences.Beijing 100093

Abstract

These results suggest that the presence of ethionine might have caused the alteration of more than one enzyme in the biosynthesis of amino acids in the selected calluses. New isoenzyme forms, 44 kD superoxide dismutase and 45 kD esterase, were found in the selected callus.

Key words: Alfalfa;Ethionine;Isoenzyme;Resistant line;Tissue culture

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引用本文：

徐云远, 贾敬芬. 苜蓿抗乙硫氨酸愈伤组织的筛选和特性[J]. 生理学报 2001; 53 (1): .

Xu Yunyuan, Jia Jingfen. Selection and characterization of cultured alfalfa calluses resistant to ethionine. Acta Physiol Sin 2001; 53 (1): (in Chinese with English abstract).