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醛固酮对心室成纤维细胞分泌内皮素的影响

龚素珍, 刘培庆, 鲁伟, 王庭槐, 符史干, 谈智, 潘敬运

中山医科大学生理学教研室.广东,广州 510089

摘要

醛固酮(1×10~(-7) mol/L)能促进心室成纤维细胞ppET-1 mRNA的表达,在作用后2h,表达开始增加,4h达高峰,以后逐渐下降;同时醛固酮能增加成纤维细胞内和条件培养液中内皮素的含量。其受体阻断剂螺旋内酯(1×10~(-6) mol/L)能拮抗醛固酮的作用,提示醛固酮能促使心室成纤维细胞分泌内皮素,且此作用是通过其受体介导的。

关键词： 醛固酮; 螺旋内酯; 内皮素放射免疫测定; ppET-1 mRNA; 心室

Effect of aldosterone on the secretion of endothelin by ventricular fibroblasts

Gong Suzhen, Liu Peiqing, Lu Wei, Wang Tinghuai, Fu Shigan, Tan Zhi, Pan Jingyun

Department of Physiology,Sun Yat-Sen University of Medical Sciences.Guangzhou 510089,Guangdong

Abstract

The results showed that aldosterone (1×10~(-7) mol/L) promoted the expression of ppET-1 mRNA, which began to increase in 2 hours and attained the highest level in 4 hours, thereafter decreased; aldosterone increased the endothelin level in ventricular fibroblasts and fibroblast conditioned growth medium (FCGM) as well, which was blocked by spironolactone (1×10~(-6) mol/L), an aldosterone receptor antagonist. The results suggest that aldosterone can increase endothelin secretion by ventricular fibroblasts, which can be inhibited by its receptor antagonist spironolactone.

Key words: aldosterone;spironolactone;radioimmunoassay for endothelin;ppET-1 mRNA;ventricular

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引用本文：

龚素珍, 刘培庆, 鲁伟, 王庭槐, 符史干, 谈智, 潘敬运. 醛固酮对心室成纤维细胞分泌内皮素的影响[J]. 生理学报 2001; 53 (1): .

Gong Suzhen, Liu Peiqing, Lu Wei, Wang Tinghuai, Fu Shigan, Tan Zhi, Pan Jingyun. Effect of aldosterone on the secretion of endothelin by ventricular fibroblasts. Acta Physiol Sin 2001; 53 (1): (in Chinese with English abstract).