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低氧预适应增强大鼠海马神经元的耐缺氧能力

赵彤, 于顺, 丁爱石, 王福庄, 范明

军事医学科学院基础医学研究所神经生物学研究室.北京 100850

摘要

本研究对整体大鼠进行了模拟不同海拔高度(3 000、5 000 m)的低氧预适应,然后观察了急性致死性缺氧对这些大鼠海马脑片诱发群峰电位的影响。结果显示,经低氧预适应的大鼠其海马脑片在给予急性缺氧后,CA1区缺氧损伤电位(hypoxic injury potential,HIP)出现时间以及突触前排放(presynaptic volley,PV)消失时间均明显延迟;其中5 000m预适应组的延迟程度比3 000m组明显。复氧后,PV的恢复率在3 000m和5 000m低氧预适应组均明显高于对照组。

关键词： 低氧; 诱发群峰电位; 海马; 耐缺氧能力

Hypoxic preconditioning enhances hypoxic tolerance of hippocampal neurons and synaptic function of rat

Zhao Tong, Yu Shun, Ding Aishi, Wang Fuzhuang, Fan Ming

Department of Neurobiology,Institute of Basic Medical Sciences.Beijing 100850

Abstract

Hypoxic preconditioning with different simulated altitudes (3 000 m and 5 000 m) was performed on Wistar rats and the evoked population spikes were recorded from the hippocampal slices of these rats. The results showed that the appearance of hypoxic injury potential (HIP) and the disappearance of presynaptic volley (PV) were significantly delayed in response to acute lethal hypoxia. HIP and PV delay became more apparent when the hypoxic preconditioning altitude was increased from 3 000 m to 5 000 m. After reoxygenation, the recovery rate of PV in hypoxic preconditioning groups at 3 000 m and 5 000 m was apparently higher than that of control. The above results suggest that hypoxic preconditioning of animals in vivo increases hypoxic tolerance of hippocampal neurons.

Key words: hypoxic preconditioning;evoked population spikes;Hippocampus;hypoxic tolerance

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引用本文：

赵彤, 于顺, 丁爱石, 王福庄, 范明. 低氧预适应增强大鼠海马神经元的耐缺氧能力[J]. 生理学报 2001; 53 (1): .

Zhao Tong, Yu Shun, Ding Aishi, Wang Fuzhuang, Fan Ming. Hypoxic preconditioning enhances hypoxic tolerance of hippocampal neurons and synaptic function of rat. Acta Physiol Sin 2001; 53 (1): (in Chinese with English abstract).