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pp60c-src在血管平滑肌细胞内丝裂原活化蛋白激酶激活中的作用

刘东, 吕俊升, 尹小龙

浙江大学医学院附属第二医院心内科.浙江,杭州 310006

摘要

将合成的反义c-src寡脱氧核苷酸(oligodeoxynucleotides,ODNs)以脂质体包裹转染培养的大鼠VSMCs,用Western印迹测得细胞裂解液中pp60c-src含量明显下降,免疫沉淀方法测得pp60c-src激酶活性仅为对照的14.4%,而同时液闪测得MAPK活性无显著性变化(P>0.05)。以AngⅡ刺激经转染反义ODNs的低pp60c-src含量与激酶活性而MAPK活性无显著性变化的VSMCs后,测得MAPK活性的增幅仅为对照的1.6%。由此可见,AngⅡ诱导VSMCs内MAPK的激活明显依赖于pp60c-src,后者在VSMCs异常增殖中可能是一个重要的信号分子。

Role of pp60c-src in mitogen-activated protein kinase activation of vascular smooth muscle cells

Liu Dong, Lu Junsheng, Yin Xiaolong

Department of Cardiology,The Second Affiliated Hospital to Zhejiang University Medical School.Hangzhou 310006,Zhejiang

Abstract

The effect of pp60c-src on mitogen-activated protein kinase (MAPK) activation induced by angiotensin II (Ang II) in vascular smooth muscle cells (VSMCs) was observed to explore the signal transduction process of VSMCs proliferation mediated by Ang II. Following transfection of the synthetic antisense c-src oligodeoxynucleotides (ODNs) wrapped in lipofectin into cultured rat VSMCs, pp60c-src content markedly decreased, pp60c-src kinase activity was only 14.4% of control, while MAPK activity was not significantly changed (P>0.05). When these transfected VSMCs were further stimulated by Ang II, the increase in MAPK activity was only 1.6% of control. These data suggest that pp60c-src is needed for MAPK activation in VSMCs stimulated by Ang II, and pp60c-src may be an important signaling molecule in abnormal proliferation of VSMCs.

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引用本文：

刘东, 吕俊升, 尹小龙. pp60c-src在血管平滑肌细胞内丝裂原活化蛋白激酶激活中的作用[J]. 生理学报 2000; 52 (6): .

Liu Dong, Lu Junsheng, Yin Xiaolong. Role of pp60c-src in mitogen-activated protein kinase activation of vascular smooth muscle cells. Acta Physiol Sin 2000; 52 (6): (in Chinese with English abstract).