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垂体腺苷酸环化酶激活肽减轻谷氨酸对海马神经元的损伤并抑制胞内钙升高

董艳, 唐铁山, 路长林, 何成, 董建波, 黄秀英, 孙方臻, 鲍xuan

第二军医大学神经生物学教研室.上海 200433；中科院发育生物学研究所.北京 100101；中科院上海脑研究所.上海 200031

摘要

对原代培养7-9d的海马神经元给予谷氨酸处理,24h后,神经元的存活率降低。预先给予垂体腺苷酸环化酶激活肽(PACAP)能显著减少谷氨酸引起的海马神经元死亡。谷氨酸呈剂量依赖性增加海马神经元细胞内钙离子含量,PACAP能抑制谷氨酸引起的海马神经元细胞内钙离子浓度的升高,特异性PACAPⅠ型受体拮抗剂PACAP 6-38能完全阻断PACAP减轻谷氨酸所致海马神经元损伤及降低谷氨酸所致神经元细胞内钙离子浓度升高的作用。

Pituitary adenylate cyclase activating polypeptide ameliorates the damage and inhibits the increase of intracellular calcium concentration in cultured hippocampal neurons induced by glutamate

Dong Yan, Tang Tieshan, Lu Changlin, He Cheng, Dong Jianbo, Huang Xiuying, Sun Fangzhen, Bao Xuan

Department of Neurobiology,Second Military Medical University.Shanghai 200433；China；Shanghai Institute of Brain Research,Chinese Academy of Sciences.Shanghai 200031

Abstract

Hippocampal neurons cultured from 7 to 9 d in vitro were used to observe the effect of glutamate. Treatment of glutamate for 24 h greatly decreased neuronal survival and pretreatment with pituitary adenylate cyclase activating polypeptide (PACAP) significantly atenuated hippocampal neuron death induced by glutamate. Moreover, glutamate dose-dependently increased the intracellular calcium concentration in cultured hippocampal neurons, while PACAP inhibited the increase of intracellular calcium concentration induced by glutamate. PACAP 6-38, a specific PACAP type I receptor antagonist, completely inhibited the amelioration of glutamate induced death and the decrease of intracellular calcium concentration induced by PACAP in cultured hippocampal neurons.

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引用本文：

董艳, 唐铁山, 路长林, 何成, 董建波, 黄秀英, 孙方臻, 鲍xuan. 垂体腺苷酸环化酶激活肽减轻谷氨酸对海马神经元的损伤并抑制胞内钙升高[J]. 生理学报 2000; 52 (5): 402-406.

Dong Yan, Tang Tieshan, Lu Changlin, He Cheng, Dong Jianbo, Huang Xiuying, Sun Fangzhen, Bao Xuan. Pituitary adenylate cyclase activating polypeptide ameliorates the damage and inhibits the increase of intracellular calcium concentration in cultured hippocampal neurons induced by glutamate. Acta Physiol Sin 2000; 52 (5): 402-406 (in Chinese with English abstract).