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注射热损伤大鼠纹状体mRNA的爪蟾卵母细胞对多巴胺的反应

赵永岐, 张炳烈, 王鲁明, 邢成, 李敏, 范明

军事医学科学院基础医学研究所.北京 100850；解放军总医院老年医学研究所.北京 100853

摘要

将从正常大鼠和热损伤大鼠的中枢纹状体提取的poly(A)~(+) mRNA,注入非洲爪蟾卵母细胞表达。用电生理方法检测多巴胺诱发的膜电位和电流的变化,分析热损伤对中枢多巴胺受体表达的影响。结果表明,注射大鼠纹状体mRNA后,卵母细胞的静息电位与注射前没有变化,但多巴胺能诱发膜电流。经验证,此受体电流的主要载流离子是Cl~(-)。注射热损伤大鼠纹状体mRNA的卵母细胞对多巴胺反应的敏感性降低,与正常大鼠组相比有显著性差异。

Responses induced by dopamine in Xenopus laevis oocytes injected with mRNA from heat injured rat striatum

Zhao Yongqi, Zhang Binglie, Wang Luming, Xing Cheng, Li Min, Fan Ming

Institute of Basic Medical Sciences,Academy of Military Medical Sciences.Beijing 100850；China

Abstract

Poly(A)~(+) mRNA isolated from the striatum of normal rats and heat injured rats by RNAgents Total RNA Isolation System and PolyTract mRNA RNAgents Isolation Systems was microinjected into Xenopus laevis ocytes to express functional neurotransmitter receptors. Membrane currents were recorded by the conventional patch clamp technique for measuring the relative amounts of mRNA encoding dopamine receptor in order to study the change in dopamine receptors in heat injured rat striatum. There was no change in resting membrane potentials of the oocytes before and after mRNA injection. Dopamine induced membrane currents were shown to be mediated through Cl~(-). The oocytes injected with mRNA from heat injured rat striatum were less sensitive to dopamine than those injected with mRNA from normal rats (P<0.05).

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引用本文：

赵永岐, 张炳烈, 王鲁明, 邢成, 李敏, 范明. 注射热损伤大鼠纹状体mRNA的爪蟾卵母细胞对多巴胺的反应[J]. 生理学报 2000; 52 (4): .

Zhao Yongqi, Zhang Binglie, Wang Luming, Xing Cheng, Li Min, Fan Ming. Responses induced by dopamine in Xenopus laevis oocytes injected with mRNA from heat injured rat striatum. Acta Physiol Sin 2000; 52 (4): (in Chinese with English abstract).