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E3泛素连接酶Cbl-b对白细胞介素-1诱导细胞效应的负调控作用

于江天, 卜歆, 赵虎, 苏金^*

第四军医大学生物化学与分子生物学教研室， 西安 710032

摘要

本研究旨在探讨E3泛素连接酶Cbl-b对滑膜细胞中白细胞介素1 (interleukin-1, IL-1)信号通路的影响。采用Western blot方法检测Cbl-b基因缺失小鼠滑膜细胞中Cbl-b及IL-1诱导的基质金属蛋白酶13 (matrix metalloproteinase 13, MMP-13)的表达水平。胶原底物与滑膜细胞培养上清进行共孵育后通过SDS-PAGE分析胶原的降解情况。结果显示，与野生型相比，Cbl-b基因敲除的小鼠滑膜细胞在IL-1刺激条件下表达更多的MMP-13，且培养上清具有更强的降解胶原的能力。这些结果提示，Cbl-b对IL-1诱导的细胞效应具有负调控作用。

关键词： Cbl-b; 泛素连接酶; 白细胞介素1; 基质金属蛋白酶13; 滑膜细胞

分类号：R392.11

[Suppression of E3 ubiquitin ligase Cbl-b in interleukin-1 signaling.] [Article in Chinese]

YU Jiang-Tian, BU Xin, ZHAO Hu, SU Jin^*

Department of Biochemistry and Molecular Biology, The Fourth Military Medical University, Xi’an, 710032

Abstract

The present study aims to investigate the effect of Cbl-b, a member of E3 ubiquitin ligase family, on interleukin-1 (IL-1) pathway in synoviocytes. The protein expression levels of Cbl-b and IL-1-induced matrix metalloproteinase 13 (MMP-13) in synoviocytes were analyzed by Western blot. Collagen substrates were incubated with the conditioned medium collected from synoviocytes cultures and then subjected to SDS-PAGE for analysis of collagen degradation. The results showed that compared with wild-type cells, Cbl-b-deficient cells expressed more MMP-13 protein and had enhanced ability to degrade collagens under IL-1 stimulation. These data suggest that Cbl-b may negatively regulate IL-1-triggered degradation of collagen matrix in synoviocytes.

Key words: Cbl-b; ubiquitin ligase; interleukin-1; matrix metalloproteinase; synoviocytes

收稿日期：2015-06-15　　录用日期：2015-07-08

通讯作者：苏金　　E-mail: sujin923@fmmu.edu.cn

引用本文：

于江天, 卜歆, 赵虎, 苏金. E3泛素连接酶Cbl-b对白细胞介素-1诱导细胞效应的负调控作用[J]. 生理学报 2015; 67 (4): 409-412.

YU Jiang-Tian, BU Xin, ZHAO Hu, SU Jin. [Suppression of E3 ubiquitin ligase Cbl-b in interleukin-1 signaling.] [Article in Chinese]. Acta Physiol Sin 2015; 67 (4): 409-412 (in Chinese with English abstract).