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GABA和孕酮对人及豚鼠精子的体外获能作用

张振汉, 陈文颖, 石其贤, 袁玉英, 楼云曹

浙江省医学科学院.浙江,杭州 310013；杭州制药厂.浙江,杭州 310029

摘要

GABA和P_(4)可明显地增加精子AR,这种作用可被GABA_(A)受体激动剂muscimol所替代。GABA所致的获能作用不仅可被GABA_(A)受体拮抗剂(-)荷包牡丹碱和印防己苦毒素所阻断,而且可被Ca~(2+)螯合剂EGTA所抑制。GABA具有明显地增强P_(4)对精子的获能作用,并可明显地激发豚鼠精子的HAM。

Capacitating action of GABA and progesterone in spermatozoa of human and guinea pig in vitro

Zhang Zhenhan, Chen Wenying, Zhi Qixian, Yuan Yuying, Lou Yuncao

Zhejiang Academy of Medical Sciences.Hangzhou 310013,Zhejiang；China

Abstract

The results showed that addition of GABA or P_(4) at 5 #mu#mol/L to the incubation medium resulted in a significant increase in the sum of "B" (characteristic of capacitated cell) and "AR" (acrosome recation) pattern (65.9% and 61.7% respectively), corresponding to capacitated spermatozoa in human, as compared to the control (37.3%). Likewise, the capacitating effect of GABA on spermatozoa in guinea pigs showed a concentration-dependent increase from 1 to 10 #mu#mol/L (AR: 27.0±1.9% to 51.6±2.8%). In addition, P_(4) potentiated the capacitating effect of GABA when combined with GABA in the capacitation stage. The capacitating effect of GABA was mimicked by a GABA_(A) receptor agonist muscimol. However, this effect was completely blocked by GABA_(A) receptor antagonist (-) -bicuculline and a GABA_(A)/Cl~(-) receptor antagonist picrotoxin. Furthermore, GABA markedly increased the HAM of P_(4) on guinea pig spermatozoa, which was mediated obviously by the influx of extracellular Ca~(2+) because the GABA-induced AR could be prevented by EGTA.

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引用本文：

张振汉, 陈文颖, 石其贤, 袁玉英, 楼云曹. GABA和孕酮对人及豚鼠精子的体外获能作用[J]. 生理学报 2000; 52 (3): .

Zhang Zhenhan, Chen Wenying, Zhi Qixian, Yuan Yuying, Lou Yuncao. Capacitating action of GABA and progesterone in spermatozoa of human and guinea pig in vitro. Acta Physiol Sin 2000; 52 (3): (in Chinese with English abstract).