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肝刺激因子对人肝癌细胞BEL-7402 p21~(ras)表达的影响

戴杰, 安威, 高鼎成, 陈莉

首都医科大学细胞生物学系.北京 100054

摘要

从雄性初断乳SD大鼠肝匀浆中提取肝刺激因子(HSS)并加以部分纯化,观察其促人肝癌细胞增殖活性及对p21~(ras)蛋白表达的影响。结果表明:(1)HSS具有明显的促人肝癌细胞增殖活性,其分子量为14-20kD;(2)HSS可提高p21~(ras)蛋白表达,具有时间-效应关系,并与EGF呈协同作用;(3)HSS调节p21~(ras)蛋白表达具有剂量-效应关系,且呈现出饱和性。

Influence of hepatic stimulator substance and p21~(ras) expression in human hepatic carcinoma cells BEL-7402

Dai Jie, An Wei, Gao Dingcheng, Chen Li

Department of Cell Biology,Capital University of Medical Sciences.Beijing 100054

Abstract

The hepatic stimulator substance (HSS) was partially purified from schedule-lighted weanling rat livers. The effects of HSS and p21~(ras) expression of human hepatic carcinoma cell BEL-7402 were examined. The results showed: (1) HSS was capable of promoting the proliferation of the cells, and its molecular weight was 14-20 kD; (2) the p21~(ras) expression was increased under HSS effect time-dependently, and the effect of HSS appeared synergistic with EGF; (3) the p21~(ras) expression induced by HSS manifested a dose-dependent manner, and the saturation effect of HSS occurred at the concentration of 100 #mu#g/ml. Considering together with our previous report about HSS regulation on EGF receptor, these results strongly suggest that the proliferatory effect of HSS on hepatic carcinoma cells is presumedly related with eGF receptor-mediated signal transduction.

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引用本文：

戴杰, 安威, 高鼎成, 陈莉. 肝刺激因子对人肝癌细胞BEL-7402 p21~(ras)表达的影响[J]. 生理学报 2000; 52 (3): .

Dai Jie, An Wei, Gao Dingcheng, Chen Li. Influence of hepatic stimulator substance and p21~(ras) expression in human hepatic carcinoma cells BEL-7402. Acta Physiol Sin 2000; 52 (3): (in Chinese with English abstract).