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白介素-2促进RC-4B/C垂体瘤细胞系增殖

王高峰, 朱运龙, 陈健康, 张万会, 钟延清, 胡玉珍, 王复周

第四军医大学基础部生理学教研室.陕西,西安 710032

摘要

IL-2(10-1 000U/ml)剂量依赖性地显著提高RC-4B/C细胞~(3)H-TdR掺入率。特异性酪氨酸蛋白激酶(PTK)抑制剂tyrphostin(1#mu#mol/L)可明显降低RC-4B/C细胞~(3)H-TdR掺入率,并可部分阻断IL-2的促细胞增殖作用。特异性蛋白激酶A(PKA)抑制剂H-9(1#mu#mol/L)作用后,~(3)H-TdR掺入率明显升高;H-9还可加强IL-2的促细胞增殖作用。在实验中未观察到抗雌激素tamoxifen(1#mu#mol/L)对IL-2促RC-4B/C细胞增殖效应有明显影响。

Interleukin-2 stimulates the proliferation of cultured RC-4B/C pituitary adenoma cell line

Wang Gaofeng, Zhu Yunlong, Chen Jiankang, Zhang Wanhui, Zhong Yanqing, Hu Yuzhen, Wang Fuzhou

The Fourth Military Medical University.Xi^an 710032,Shaanxi

Abstract

IL-2 (10-1 000 U/ml) significantly stimulated ~(3)H-TdR incorporation into the cell line in a dose-dependent manner. Specific PTK inhibitor tyrphostin (1 #mu#mol/L) suppressed RC-4B/C cell proliferation and blocked the effect of IL-2 on RC-4B/C cells. After the PKA signaling pathway was inhibited by specific PKA inhibitor H-9 (1#mu#mol/L), the proliferation rate of RC-4B/C cells increased significantly. H-9 also enhanced the stimulation of IL-2 on RC-4B/C cell growth. Anti-estrogen tamoxifen (1#mu#mol/L) had no significant effect on the action of IL-2 on the proliferation of RC-4B/C cells.

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引用本文：

王高峰, 朱运龙, 陈健康, 张万会, 钟延清, 胡玉珍, 王复周. 白介素-2促进RC-4B/C垂体瘤细胞系增殖[J]. 生理学报 2000; 52 (3): .

Wang Gaofeng, Zhu Yunlong, Chen Jiankang, Zhang Wanhui, Zhong Yanqing, Hu Yuzhen, Wang Fuzhou. Interleukin-2 stimulates the proliferation of cultured RC-4B/C pituitary adenoma cell line. Acta Physiol Sin 2000; 52 (3): (in Chinese with English abstract).