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三种钠尿肽抑制大鼠肺动脉平滑肌细胞增殖效应的比较

董明清, 朱妙章, 于军, 商立军, 冯华松

第四军医大学生理学教研室.陕西,西安 710032；第四军医大学唐都医院呼吸科.陕西,西安 710032

摘要

比较了心房钠尿肽(ANP)、C-型钠尿肽(CNP)、血管钠肽(VNP)抑制肺动脉平滑肌细胞(PASMCs)增殖的效应。用蛋白激酶C激动剂佛波酯(PMA)刺激体外培养大鼠PASMCs的增殖,以总蛋白含量和MTT比色OD值为指标,观察三种钠尿肽对PMA刺激大鼠PASMCs增殖的影响。结果表明,PMA(10~(-9)-10~(-7) mol/L)显著升高(P<0.05)PASMCs的总蛋白含量和MTT OD值,VNP(10~(-8)-10~(-6) mol/L)、ANP和CNP(10~(-7)-10~(-6) mol/L)均可显著降低(P<0.05)PMA(10~(-8) mol/L)刺激PASMCs的总蛋白含量和MTT OD值。

Comparison of inhibitory effects of three natriuretin peptides on the proliferation of pulmonary artery smooth muscle cells of rats

Dong Mingqing, Zhu Miaozhang, Yu Jun, Shang Lijun, Feng Huasong

Department of Physiology,Fourth Military Medical University.Xi^an 710032,Shaanxi；China

Abstract

The inhibitory effects of atrial natriuretic peptide (ANP), C-type natriuretic peptide (CNP) and vasonatrin peptide (VNP) on the proliferation of pulmonary artery smooth muscle cells (PASMCs) were compared in rats. Total protein and OD value of MTT were detected in cultured rat PASMCs to examine the influence of the three peptides on PASMC proliforation induced by phorbol 12-myristate 13-acetate (PMA). The results show that PMA (10~(-9)-10~(-7) mol/L) increased and VNP (10~(-8)-10~(-6) mol/L), ANP and CNP (10~(-7)-10~(-6) mol/L) decreased the total protein and OD value in a dose-dependent manner (P<0.05).

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引用本文：

董明清, 朱妙章, 于军, 商立军, 冯华松. 三种钠尿肽抑制大鼠肺动脉平滑肌细胞增殖效应的比较[J]. 生理学报 2000; 52 (3): .

Dong Mingqing, Zhu Miaozhang, Yu Jun, Shang Lijun, Feng Huasong. Comparison of inhibitory effects of three natriuretin peptides on the proliferation of pulmonary artery smooth muscle cells of rats. Acta Physiol Sin 2000; 52 (3): (in Chinese with English abstract).