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褪黑素减弱大鼠下丘脑弓状核#beta#-内啡肽免疫反应的强度

俞昌喜, 吴根诚, 许绍芬, 陈崇宏

上海医科大学神经生物学教研室,医学神经生物学国家重点实验室.上海 200032；福建医科大学药理学教研室.福建,福州 350004

摘要

采用免疫组化方法结合计算机图像处理技术,观察了注射MEL对大鼠下丘脑弓状核内神经细胞的#beta#-内啡肽免疫反应的影响。实验大鼠分给药组及对照组,分别腹腔注射MEL 110mg/kg或配药液,1h后灌注取脑、冰冻切片,进行免疫组化染色,计算机图像处理技术测定染色脑片积分光密度(IOD)和平均光密度(OD)。结果显示,给药组大鼠弓状核内#beta#-内啡肽免疫反应明显减弱,IOD及OD也均显著减少(P<0.01;P<0.05),表明MEL可致弓状核内#beta#-内啡肽含量减少,系MEL促进#beta#-内啡肽释放所致,从而发挥镇痛效应。

Melatonin attenuates the intensity of #beta#-endorphin immunoreactivity in the arcuate nucleus of rat hypothalamus

Yu Changxi, Wu Gencheng, Xu Shaofen, Chen Chonghong

State Key Laboratory of Medical Neurobiology,Department of Neurobiology,Shanghai Medical University.Shanghai 200032；China

Abstract

The results suggest that melatonin may result in a decrease of #beta#-Ep content in the arcuate nucleus, as a result of increased #beta#-Ep release induced by administration of melatonin. It is likely that the analgesic effect of melatonin may be in part mediated by the release of #beta#-endorphin from the arcuate nucleus.

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引用本文：

俞昌喜, 吴根诚, 许绍芬, 陈崇宏. 褪黑素减弱大鼠下丘脑弓状核#beta#-内啡肽免疫反应的强度[J]. 生理学报 2000; 52 (3): .

Yu Changxi, Wu Gencheng, Xu Shaofen, Chen Chonghong. Melatonin attenuates the intensity of #beta#-endorphin immunoreactivity in the arcuate nucleus of rat hypothalamus. Acta Physiol Sin 2000; 52 (3): (in Chinese with English abstract).