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褪黑素对大鼠中脑导水管周围灰质内阿片肽释放的影响

俞昌喜, 吴根诚, 许绍芬, 陈崇宏

上海医科大学神经生物学教研室、医学神经生物学国家重点实验室.上海 200032；福建医科大学药理学教研室.福建,福州 350004

摘要

采用推挽灌流技术、放射免疫测定法,观察褪黑素(melatonin,MEL)对大鼠中脑导水管周围灰质(PAG)推挽灌流液中#beta#-内啡肽(#beta#-Ep)、亮氨酸脑啡肽(L-EK)含量的影响,以探讨MEL镇痛效应的中枢机制。结果显示,给药组大鼠腹腔注射(ip)MEL 110mg/kg后30-50min,PAG灌流液中#beta#-Ep含量显著增加,而L-EK含量未见显著变化;在推挽灌流同时用50℃热水刺激甩尾法测定痛阈,可见ip MEL 110mg/kg后40min,大鼠痛阈显著升高。

关键词： 褪黑素; β-内啡肽; 脑啡肽; 中脑导水管周围灰质; 镇痛; 大鼠

Melatonin influences the release of endogenous opioid peptides in rat periaqueductal gray

Yu Changxi, Wu Gencheng, Xu Shaofen, Chen Chonghong

State Key Laboratory of Medical Neurobiology,Department of Neurobiology,Shanghai Medical University.Shanghai 200032；China

Abstract

The present study was undertaken to explore central mechanisms underlying the analgesic effect of melatonin. Push-pull perfusion technique and radioimmunoassay were used to observe the changes in the contents of #beta#-endorphin (#beta#-Fp) and leucine0enkephalin (L-EK) in the perfusate from the rat periaqueductal gray (PAG) after administration of melatonin. 30-50 min after an intraperitoneal injection of melatonin (110mg/kg), the #beta#-Ep content in the perfusate was increased significantly, while the L-EK content was not changed. Pain threshold was measured using the warm water tail-flick test during the push-pull perfusion of the PAG. It was found that the rat pain threshold was increased significantly 40 min after the intraperitoneal injection of melatonin (110mg/kg).

Key words: Melatonin;#beta#-endorphin;Enkephalin;Periaqueductal gray;Analgesia;Rat

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引用本文：

俞昌喜, 吴根诚, 许绍芬, 陈崇宏. 褪黑素对大鼠中脑导水管周围灰质内阿片肽释放的影响[J]. 生理学报 2000; 52 (3): .

Yu Changxi, Wu Gencheng, Xu Shaofen, Chen Chonghong. Melatonin influences the release of endogenous opioid peptides in rat periaqueductal gray. Acta Physiol Sin 2000; 52 (3): (in Chinese with English abstract).