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bFGF与BDNF对损伤后成年小鼠离体培养嗅觉上皮细胞发育的影响

张晓东, 郭占芳, 刘宁, Roisen F J

苏州大学生物技术中心.江苏,苏州 215151；美国路易斯维尔大学医学院解剖科学和神经生物学系.美国

摘要

将bFGF与BDNF的组合分别加到10%和5%血清的OE培养基(OEM)中,增加了双极细胞的数量和比例,刺激了双极细胞突起的发育,减少了大细胞和梭形细胞。这种组合的效果比bFGF或BDNF单独作用好。在10%血清的OEM中,神经营养因子的效果比在5%血清的OEM中好。

关键词： 嗅觉上皮; 碱性成纤维细胞生长因子; 脑源神经营养因子; 免疫细胞化学

Effects of bFGF and BDNF on the cells of injured adult mouse olfactory epithelium in vitro

Zhang Xiaodong, Guo Zhanfang, Liu Ning, Roisen F J

Biotechnological Center,Suzhou University.Suzhou 215151,Jiangsu；USA

Abstract

The results showed that the combination of bFGF and BDNF added to 10% and 5% serum OE medium increased the bipolar cell number and percentage, stimulated the process outgrowth of bipolar cells, and decreased the numbers of giant cells and fusiform cells. The effects of the combination of bFGF and BDNF in the 10% serum OE medium were more significant than those of bFGF and BDNF applied alone; and the effects of neurotrophic factors in the 10% serum OE medium were more significant than those observed in the 5% serum OE medium.

Key words: Olfactory epithelium;Basic fibroblast growth factor;Brain derived neurotrophic factor;Immunocytochemistry

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引用本文：

张晓东, 郭占芳, 刘宁, Roisen F J. bFGF与BDNF对损伤后成年小鼠离体培养嗅觉上皮细胞发育的影响[J]. 生理学报 2000; 52 (3): .

Zhang Xiaodong, Guo Zhanfang, Liu Ning, Roisen F J. Effects of bFGF and BDNF on the cells of injured adult mouse olfactory epithelium in vitro. Acta Physiol Sin 2000; 52 (3): (in Chinese with English abstract).