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电子束辐射对大麦种胚核酸合成活性的损伤效应

刘晓, 赵玉芳, 凌备备

中科院大连化学物理研究所.辽宁,大连 116023；浙江大学原子核农业科学研究所.浙江,杭州 310029

摘要

电子束辐射损伤大麦种胚核酸合成能力的效应主要表现在使DNA复制合成启动推迟,使DNA复制、RNA合成活性下降。吸涨过程中大麦种胚的DNA复制合成有一明显的启动过程,RNA合成则无明显的启动过程。200与400Gy电子束辐射分别使DNA复制合成启动推迟约2与4h;电子束辐射对DNA复制合成活性的抑制作用强于对RNA合成活性的抑制。在50-500Gy范围内,大麦种胚DNA复制、RNA合成活性均随辐射剂量呈指数下降,其半对数斜率分别为-0.0039与-0.0014。根据实验计算,电子束辐射对DNA复制合成的LD_(50)、D_(37)分别为178与256Gy。

关键词： 电子束辐射; DNA复制; RNA合成; 大麦

Lowering of barley embryo nucleic acid synthesis activity induced by electron beam irradiation

Liu Xiao, Zhao Yufang, Ling Beibei

Dalian Institute of Chemical Physics,Chinese Academy of Sciences.Dalian 116023,Liaoning；China

Abstract

The lowering effect of EB-irradiation on DNA replication activity was much stronger than that on RNA synthesis, with the slopes of their semilogarithmic curves being -0.0039 and -0.0014 respectively. According to this experiment, the 50% inhibitory dosage and lethal dosage of electron beam for DNA synthesis activity in barley embryos were about 178 and 256 Gy respectively.

Key words: Electron beam irradiation;DNA replication;RNA synthesis;Barley

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引用本文：

刘晓, 赵玉芳, 凌备备. 电子束辐射对大麦种胚核酸合成活性的损伤效应[J]. 生理学报 2000; 52 (3): .

Liu Xiao, Zhao Yufang, Ling Beibei. Lowering of barley embryo nucleic acid synthesis activity induced by electron beam irradiation. Acta Physiol Sin 2000; 52 (3): (in Chinese with English abstract).